Leica Sp5
Information about the Leica Sp5.
Available Lasers
| Laser Line | Power at x20 objective |
| 405nm | 0.7mW |
| 458nm | NA |
| 488nm | 10% of maximum power 0.8mW |
| 514nm | 10% of maximum power 0.3mW |
| 561nm | 1.6mW |
| 594nm | 0.3mW |
| 633nm | 2.1mW |
Software
- Leica LAS-AF (Confocal)
- ISS Vista (FCS)
Available Modules
- Conventional and Resonant Scanners
- 5 detection channels (2xHyD)
- Spectrometers (all visible wavelengths)
- FRAP, FRET
- FCS,FCCS
- 8K x 8K image size on conventional scanner
- 25 frames/sec at 512x512 using resonant scanner
Objectives
| Type | Magnification | Correction | NA |
| HCX PLAN APO | x20 | Oil, Water, Lbd Bl | 0.7 |
| HCX PLAPO | x40 | Oil, PH3, CS | 1.25 |
| HCX PLAPO | x63 | W Corr Lbd Bl | 1.2 |
| HCX PLAPO | x63 | Glycerol | 1.3 |
| HCX PLAPO | x63 | Oil CS | 1.4 |
| HCX PLAPO | x100 | Oil | 1.46 |
Supporting Equipment
- Environmental chamber
- Temperature control from ambient to 45°C
- Humidified CO2 concentration 1% to 11%
Fluorescence Correlation Spectroscopy (FCS)
- Single pixel beam positioning
- Dual channel for Fluorescence Cross Correlation Spectroscopy (FCCS)
- 3 Filter Sets available
- GFP/RFP - Band Pass filters 500-550nm and 607-683nm, Beam Splitter 560nm
- CFP/YFP - Band Pass filters 470-500nm and 535-585nm, Beam Splitter 505nm
- Alexa 488/633 - Band Pass filters 500-550nm and 647-703nm, Beam Splitter 625nm
Room Location
Swann 2.15