Spinning Disk Confocal
Information about Spinning Disk Confocal equipment.
Available Lasers
| Laser | Power at x20 Objective |
| 488nm | 1.45mW |
| 514nm | 1.01mW |
| 561nm | 1.58mW |
| 594nm | 1.45mW |
Available Filter Sets
| Fluorophore | Excitation | Dichroic | Emission | Filter Set Choices |
| eGFP | 488 |
488/561 488/594 |
525/50 |
Dichroic: Chroma zt488/561tpc Dichroic: Chroma zt488/594tpc Emission: Chroma ET525/50m |
| YFP | 514 | 514/594 | 545/40 |
Dichroic Semrock Di01-T445/514/593 Emission: Chroma: ET545/40m |
| tdTomato | 564 | 488/561 | 590LP |
Dichroic: Chroma zt488/561tpc Emission: Chroma T590lpxr-UF2 |
| mCherry | 594 |
514/594 488/594 |
645/75 |
Dichroic Semrock Di01-T445/514/593 Dichroic Chroma zt488/594tpc Emission: Chroma ET645/75m |
Software
- Metamorph
Available Modules
- 4D Acquistion
- ASI Crisp autofocus system for long term timelapse
- ASI piezo XYZ stage for high accuracy/repeatability
- Andor iXon EMCCD camera
- Yokogawa spinning disk unit
- iLAS FRAP module (GFP only)
Objectives
| Type | Magnification | Correction | NA |
| Plan Apo | x20 | Air, DIC | 0.75 |
| Plan Fluor | x40 | Air | 0.75 |
| Plan Apo TIRF | x100 | Oil, DIC | 1.45 |
Supporting Equipment
- Environmental Chamber
- Temperature control range from ambient to 45°C
- Humidified CO2 from 1% to 11% available on request
- Optisplit
- Simultaneous acquisition of upto 3 fluorophores.
- Splits camera chip into 2 or 3 regions.
Room Location
Swann 2.15