Deltavision Elite
There are 2 Deltavision Elites in the Light Microscopy Core.
The Deltavision Elites are equipped with:
Filter Sets
| Fluorophore | Excitation | Dichroic | Emission |
|---|---|---|---|
| DAPI | 395/25 | ||
| CFP | 434/21 | ||
| GFP/FITC | 485/25 | ||
| YFP | 511/16 | ||
| Rhodamine/tdTomato | 560/32 | ||
| Texas Red/mCherry | 585/35 | ||
| Cy5 | 631/28 |
Software
- SoftWoRx 3.5
Available Modules
- Ultimate focus system for long term timelapse studies.
- High accuracy/repeatability motorised stage for multipoint imaging.
- Photometrics Cascade II EMCCD or Photometrics Coolsnap CCD
- FRAP and Photoactivation module
Objectives
| Type | Magnification | Correction | NA |
|---|---|---|---|
| UPlanApo | x10 | Air | 0.4 |
| UApo/340 | x20 | Air | 0.75 |
| UApo/340 | x40 | Oil | 1.35 |
| PlanApoN | x60 | Oil | 1.42 |
| UPlanSApo | x60 | Water | 1.2 |
| uPlanSApo | x100 | Oil | 1.4 |
Supporting Equipment
- Cellasics Onix Microfluidics System
- Currently takes plates for yeast and bacteria
- Enables multiple parallel experiments
- Upto 6 wells for media changes and addition of compounds/chemicals during experiments.
- Scientifica PTC-20 2 channel heating/cooling controller
- Temperature control 3°C to 45°C
- Environmental Chamber
- Temperature range from ambient to 45°C
- Humidified CO2 from 1% to 11% on request
Room Location
Both Deltavision Elite microscopes are in Swann 2.16