Zeiss LSM 880 (Airyscan)
Information about Zeiss LSM 880 with Fast Airyscan equipment.
Available Laser Lines
Laser Type | Line | Maximum Power at x20 Objective |
Diode | 405nm | 4.88mW |
Argon | 458nm | NA |
488nm | 2.64mW | |
514nm | 1.04mW | |
HeNe | 561nm | 6.82mW |
HeNe | 594nm | 0.88mW |
Diode | 633nm | 1.74mW |
Software
- Zen Black (on microscope)
- Zen Black (Off line)
- Zen Blue 2.3 (Off Line)
Available Modules
- Fluorescence Recovery after Photobleaching (FRAP)
- Fluorescence Correlation Spectroscopy(FCS)
- 4D imaging (Time,X,Y and Z)
- Definite Focus
- Colocalisation
- Spectral Unmixing
- Tiles & Positions
- XY scanning stage
- 2 x PMT's detectors
- 1 x GaAsp detector
- Airyscan detector (120nm XY and 350nm Z)
Objectives
Type | Magnification | Correction | NA |
Plan Apochromat | x10 | Air | 0.45 |
Plan Apochromat | x20 | Air | 0.8 |
Plan Apochromat | x40 | Oil | 1.3 |
Plan Apochromat | x63 | Oil | 1.4 |
Alpha Plan Apochromat | x100 | Oil | 1.4 |
Airyscan (with Fast module, 25FPS)
Suggested Laser Line | Emission Filter |
405nm 488nm | BP 420-480 + BP 495-550 |
488nm, 561nm | BP 495-550 + LP 570 |
561nm, 633nm | BP 570-620 + LP 645 |
405nm, 488nm, 561nm, 594nm | BP 420-480 + BP 495-620 |
405nm, 458nm, 488nm, 514nm | BP 465-505 + LP 525 |
405nm, 561nm, 594nm, 633nm | BP 420-480 + LP 605 |
Supporting Equipment
- Environmental Chamber
- Temperature control (Ambient - 45°C)
- CO2 module (1-10%)
Room Location
Swann 2.11