Nikon Ti2
Information about Nikon Ti2 Live Imaging Microscope equipment.
Available Exciter/Emitter Combinations
| Suggested Fluorophore | SpectraX Illumination | Available Excitation Filter | Available Dichroic | Available Emission Filter |
| DAPI | 378/52 | 377/26 | 409 | 432/36 |
| CFP | 438/24 | 438/24 | 459 | 482/24 |
| GFP | 474/26 | 474/26 | 493 | 525/40 or 515/30 |
| YFP | 509/22 | 509/22 | 526 | 544/24 or 543/22 |
| Cy3/tdTomato | 554/23 | 543/23 | 573 | 596/83 or 595/31 |
| TxRed/mCherry | 575/25 | 578/21 | 596 | 641/75 or 702/197 |
| Far Red/Cy5 | 635/18 | 638/18 | 652 | 698/70 or 730/139 |
Software
Nikon Elements 5.1
Available Modules
- 4D acquisition
- Multi-stage position
- Triggered acquisition
- Perfect focus for long term timelapse
- JOBS acquisition
- Photometrics Prime 95B
Objectives
| Type | Magnification | Correction | NA |
| CFI Plan Apochromat Lambda | x20 | Air | 0.75 |
| CFI Plan Apochromat Lambda | x40 | Air | 0.95 |
| CFI Plan Apochromat Lambda | x60 | Oil | 1.3 |
| CFI Plan Apochromat TIRF | x100 | Oil | 1.49 |
Supporting Equipment
- OKOLab Environmental chamber
- Active CO2 Controller (1 to 11%)
- Temperature Controller (Ambient to 45°C)
Room Location
Swann 2.11