Zeiss Axio Observer
Information about Zeiss Axio Observer equipment.
Filter Sets
| Fluorophore | Excitation | Dichroic | Emitter | Filter Set |
| DAPI | UV: 385/30nm |
405 395 |
425/30 445/50 |
Zeiss Set 90 HE LED Zeiss Set 49 |
| CFP | V: 423/44nm | 450 | 467/24 | Zeiss Set 91 HE LED |
| GFP/FITC/Alexa 488 | B: 469/38nm |
495 493 |
525/50 514/30 |
Zeiss Set 38 HE Zeiss Set 90HE LED |
| YFP | C: 511/44nm | 538 | 555/25 | Zeiss Set 91 LED |
| RFP/tdTomato | G: 555/30nm |
570 575 |
605/70 592/25 |
Zeiss Set 43 HE Zeiss Set 90 HE LED |
| mCherry | Y: 590/27nm | 610 | 687/185 | Zeiss Set 91 LED |
| Cy 5 | R: 631/33nm | 653 | 709/100 | Zeiss Set 90 HE LED |
Software
- Zen 2.3
Available Modules
- 4D Acquisition
- Triggered Acquisition for high speed applications
- Scanning Stage for multipoint acquisitions
- Hamamatsu Orca Flash 4 V2
- Definite Focus II
Objectives
| Type | Magnification | Correction | NA |
| Plan ApoChromat | x40 | Oil, DIC | 1.4 |
| Plan ApoChromat | x60 | Oil, DIC | 1.4 |
| alpha-Plan ApoChromat | x100 | Oil, DIC | 1.46 |
Supporting Equipment
- Environmental Chamber
- Temperature Control Ambient to 45°C
- Humidified CO2 concentration 1% to 10%
Room Location
Swann 2.10