Information about Zeiss LSM 980 (Airyscan 2) equipment.
Available Lasers
| Laser |
Laser Power at x20 Objective |
| 405nm |
4.8mW |
| 488nm |
6.2mW |
| 561nm |
6.8mW |
| 594nm |
1.6mW |
| 633nm |
5.5mW |
Software
- Zen Blue 3.3 (on microscope)
- Zen Black 2.3 (Off line)
- Zen Blue 3.3 (Off Line)
Available Modules
- Fluorescence Recovery after Photobleaching (FRAP)
- Fluorescence Correlation Spectroscopy(FCS)
- 4D imaging (Time,X,Y and Z)
- Definite Focus
- Colocalisation
- Spectral Unmixing
- Tiles & Positions
- XY scanning stage
- 2 x PMT's detectors
- 1 x GaAsp detector
- Airyscan detector (120nm XY and 350nm Z)
Objectives
| Type |
Magnification |
Correction |
NA |
Contrast Method |
| Plan Apochromat |
x20 |
Air |
0.8 |
|
| Plan Apochromat |
x63 |
Water |
1.2 |
|
| Plan Apochromat |
x63 |
Oil |
1.4 |
DIC |
| Alpha Plan Apochromat |
x100 |
Oil |
1.45 |
DIC |
AiryScan 2 (with 4Y 25 frames per second)
| Suggested Laser Lines |
Emission Filter |
| 405nm 488nm |
BP420-480 BP495-550 |
| 405nm 561nm |
BP420-480 BP570-630 |
| 405nm 561nm 594nm |
BP420-500 LP605 |
| 488nm 561nm 594nm |
BP495-555 BP570-630 |
| 488nm 633nm |
BP495-555 LP660 |
| 561nm 633nm |
BP570-620 LP660 |
Supporting Equipment
- Environmental Chamber
- Temperature Control Ambient - 45ºC
- CO2 Module (1-10%)
- Slide Mounting Frame
- 35mm Petri dish frame
- Well Plate frame
Room Location
Swann 2.11
