Zeiss LSM 980 (Airyscan 2)

Information about Zeiss LSM 980 (Airyscan 2) equipment.

Available Lasers

LaserLaser Power at x20 Objective
405nm4.8mW
488nm6.2mW
561nm6.8mW
594nm1.6mW
633nm5.5mW
Photo of Zeiss LSM 980 Airyscan 2

Software

  • Zen Blue 3.3 (on microscope)
  • Zen Black 2.3 (Off line)
  • Zen Blue 3.3 (Off Line)

Available Modules

  • Fluorescence Recovery after Photobleaching (FRAP)
  • Fluorescence Correlation Spectroscopy(FCS)
  • 4D imaging (Time,X,Y and Z)
  • Definite Focus
  • Colocalisation
  • Spectral Unmixing
  • Tiles & Positions
  • XY scanning stage
  • 2 x PMT's detectors
  • 1 x GaAsp detector
  • Airyscan detector (120nm XY and 350nm Z)

Objectives

TypeMagnificationCorrectionNAContrast Method
Plan Apochromatx20Air0.8 
Plan Apochromatx63Water1.2 
Plan Apochromatx63Oil1.4DIC
Alpha Plan Apochromatx100Oil1.45DIC

AiryScan 2 (with 4Y 25 frames per second)

Suggested Laser LinesEmission Filter
405nm  488nmBP420-480  BP495-550
405nm  561nmBP420-480  BP570-630
405nm  561nm  594nmBP420-500  LP605
488nm  561nm  594nmBP495-555  BP570-630
488nm  633nmBP495-555  LP660
561nm  633nmBP570-620  LP660

Supporting Equipment

  • Environmental Chamber
  • Temperature Control Ambient - 45ºC
  • CO2 Module (1-10%)
  • Slide Mounting Frame
  • 35mm Petri dish frame
  • Well Plate frame

Room Location

March G.7

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