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Flow Cytometry

Flow Cytometry is a technique for counting and examining microscopic particles, such as cells and chromosomes, by suspending them in a stream of fluid and passing them by an electronic detection apparatus.

This allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of up to thousands of particles per second.

Flow Cytometry is routinely used in the diagnosis of health disorders, especially blood cancers, but has many other applications in both research and clinical practice.

A common variation is to physically sort particles based on their properties, so as to purify populations of interest (FACS).

The technology has applications in a number of fields, including molecular biology, pathology, immunology, plant biology and marine biology.

An introduction to Flow Cytometry is available online. These presentations will give you a basic understanding of Flow Cytometry prior to your training on the instruments.

Instruments

Details of the instruments available.

Booking

There is a calendar booking system for each of the analysis cytometers. Please only book the required period of time for analysis of your samples.

Training

The LSRFortessa and Fortessa X-20 are available for use by all researchers who have received appropriate training.

Data analysis

Please do not undertake data analysis on the instruments.

Useful Links

Related flow cytometry links and presentations