Leica DMi8 basic fluorescence widefield microscope
Leica DMi8 basic equipment details
Standard widefield microscope for fluorescence-based imaging
This system is based on an inverted Leica DMi8 microscope that is equipped with a Sola II LED light source and four standard filter cubes (see below). The camera for monochrome image acquisition is a Leica DFC3000 G with a full frame size of 1296 pixels x 966 pixels. The XY-stage is manually operated, whereas the Z-drive is motorised. The system is located is in room C2.38.
Objectives
The DMi8 is equipped with the following lenses:
|
Lens |
magnif. |
corr |
NA |
|
N PLAN |
10x |
A |
0.25 |
|
N PLAN L |
20x |
A |
0.4 |
|
HCX PL FLUOTAR L Ph2 |
40x |
A |
0.6 |
|
HCX PL APO |
63x |
O |
1.3 |
A = air, O = immersion oil
Filter sets
The following filter sets for epifluorescence illumination are available on the DMi8:
|
Fluorophore |
exc |
|
em |
|
DAPI |
405/10 |
460/40 |
|
|
FITC |
470/40 |
525/50 |
|
|
TexR |
560/40 | LP610 | |
|
Cy5 |
620/60 |
700/75 |