Confocal and Advanced Light Microscopy (CALM) Facility

Leica TCS SP8 confocal laser scanning microscope

Equipment details

This system is based on a fully motorised Leica DMi8CEL (inverted) research microscope, and the scanhead is equipped with one conventional photo multiplier tube and two highly sensitive HyD detectors. The entire microscope is controlled via the Leica LAS X software , which includes the Navigator module that is very intuitive and ideal for semi-automated image acquisition from large sample areas. The software also provides modules for spectral unmixing and ratiometric Förster Resonance Energy Transfer (FRET). The system is also equipped with a stage top environment control chamber to maintain optimal conditions for live cell imaging (temperature, carbon dioxide concentration and air humidity).

Lasers

The TCS SP8 provides the following lasers with the specified laser lines for fluorophore excitation:

Laser lines [nm]

Blue diode        

405  

   

Arg

458    

488    

514

Diode

561

   

HeNe

633

   

 

Objectives

The Leica TCS SP8  is equipped with the following lenses:

Lens

magnification        

immersion     

NA          

WD

HC PL APO 10x CS2     

10x

A

0.4

2.2

HC PL APO 20x CS2

20x

A

0.75

0.62

HC PL APO 40x CS2

40x

O

1.3

0.24

HC PL APO 63x CS2

63x

O

1.4

0.14

HC PL APO 63x

63x

W

1.2

 

 

W = water, A = air, O = oil, WD = working distance in mm

 

Filter sets for epifluorescence illumination/visual check

Standard filter cubes for epifluorescence detection of DAPI (exc 360/40, em LP425), FITC (exc 470/40, em LP515) and Rhodamine (exc 540/45, em LP590) are available on the TCS SP8.