Bovine TB Diagnostics
Developing novel diagnostic tools for tuberculosis.
Improved diagnosis of TB in humans and cattle will lead to reduction in the prevalence of disease. This team aims to develop a point-of-care diagnostic for bovine TB using samples from experimental and field infected cattle. The goal is to devise a lateral flow assay that can be used cheaply and simply in the field without the need for a veterinarian visit.
We will use extensive knowledge from human studies to guide selection of the serum components for which to test initially but will also include novel aspects for the bovine as required.
This is a joint PhD project with the University of Leiden funded for three years from February 2020.
Team members: Hamza Khalid and Tim Connelley
Team partners: Annemieke Geluk at Leiden University
Key references:
Khalid H, van Hooij A, Connelley TK, Geluk A, Hope JC. Protein Levels of Pro-Inflammatory Cytokines and Chemokines as Biomarkers of Mycobacterium bovis Infection and BCG Vaccination in Cattle. Pathogens. 2022; 11(7):738. https://doi.org/10.3390/pathogens11070738
Anouk van Hooij, Elisa M. Tjon Kon Fat, Danielle de Jong, Marufa Khatun, Santosh Soren, Abu Sufian Chowdhury, Johan Chandra Roy, Khorshed Alam, Jong-Pill Kim, Jan Hendrik Richardus, Annemieke Geluk, Paul L.A.M. Corstjens. Prototype multi-biomarker test for point-of-care leprosy diagnostics. iScience. 2021 24(1). ISSN 2589-0042. https://doi.org/10.1016/j.isci.2020.102006.
Penn-Nicholson, A., Hraha, T., Thompson, E. G., Sterling, D., Mbandi, S. K., Wall, K. M., Fisher, M., Suliman, S., Shankar, S., Hanekom, W. A., Janjic, N., Hatherill, M., Kaufmann, S., Sutherland, J., Walzl, G., De Groote, M. A., Ochsner, U., Zak, D. E., Scriba, T. J., & ACS and GC6–74 cohort study groups (2019). Discovery and validation of a prognostic proteomic signature for tuberculosis progression: A prospective cohort study. PLoS Med. 16(4), e1002781. https://doi.org/10.1371/journal.pmed.1002781
Suliman, S., Thompson, E. G., Sutherland, J., Weiner, J., 3rd, Ota, M., Shankar, S., Penn-Nicholson, A., Thiel, B., Erasmus, M., Maertzdorf, J., Duffy, F. J., Hill, P. C., Hughes, E. J., Stanley, K., Downing, K., Fisher, M. L., Valvo, J., Parida, S. K., van der Spuy, G., Tromp, G., … GC6-74 cohort study team, The ACS cohort study team (2018). Four-Gene Pan-African Blood Signature Predicts Progression to Tuberculosis. Am. J Resp. Crit. Care Med. 197(9), 1198–1208. https://doi.org/10.1164/rccm.201711-2340OC