Epifluorescence

Details of our epifluorescent systems: Macred and Limelight, Britemac, Granny, Scumpy Calvados.

Macred, Britemac and Limelight

Modality Epifluorescence

Fluorophores: DAPI, FITC, Texas Red, TRITC, Cy5 + BF

Manufacturer Zeiss AxioImager 2

Objectives 2.5x, 5x, 10x, 20x, 40x, 63x, 100x

Software Micro-Manager

Application Fixed, colour brightfield, Britemac has an x,y,z scanning stage and is capable of tiling.

Granny

Modality Epifluorescence

Fluorophores DAPI, FITC, Texas Red, TRITC, Cy5 + BF

Manufacturer Zeiss AxioImager.A1

Objectives 2.5x, 5x, 10x, 20x, 40x, 63x, 100x

Software Nikon NIS

Application Fixed, z-stack/deconvolution

Calvados and Scrumpy

Modality Epifluorescence (Inverted), Live imaging, Conditional imaging (Scrumpy)

Fluorophores DAPI, GFP, RFP, CFP, YFP + BF

Manufacturer Zeiss Observer.Z1 (Calvados). Nikon Ti (Scrumpy)

Objectives 2.5x, 5x, 10x, 20x, 40x, 63x, 100x

Software Micro-Manager (Calvados), NIS Elements (Scrumpy)

Application Fixed, brightfield, tiling, z-stack, live imaging