National Avian Research Facility
National Avian Research Facility

Genetically altered lines

The NARF has gene editing facilities for the chicken, a variety of reporter lines and a natural mutation line.

Fertile eggs and chicks are available for research use upon request, with a requirement for a material transfer agreement for some lines to meet our restrictions relating to animal experiments or the source of the lines.

Resources for gene editing

Genetically altered lines Description Facility Availability

DDX4 KO                                

Sterile female surrogate host                            

Further information          

This genetically sterile female surrogate host chicken is used for the creation of new genetically altered poultry lines.

Sterile female host birds can transmit transplanted donor primordial germ cells to 100% of their offspring.

Conventional

and SPF  

Available                                 

iCaspase9 surrogate hosts

 

Further information

The iCaspase9 line can be used for the creation of new genetically altered poultry lines. 

In the iCaspase9 surrogate host line, both male and female germ cell lineages can be chemically ablated.

Sire Dam Surrogate mating results in 100% transmission of introduced primordial germ cells in the first generation of offspring.

Conventional

and SPF

Available                                

Reporter lines

Genetically altered lines  Description Facility Availability

Roslin Green

(Cytoplasmic GFP)      

Further information                                                      

Reporter line. Cells from GFP chickens ubiquitously express cytoplasmic eGFP, allowing descendants to be traced after grafting or in cell culture.

 

Conventional Available

Membrane GFP

Further information

Reporter line. Membrane-localised eGFP.

Useful for identifying individual cell boundaries when imaging.

 

Conventional Available

Lox-STOP-lox

Further information

Reporter line. Inducible expression of eGFP by CRE-mediated recombination, either by CRE protein application or injection of a CRE-expressing plasmid.

This allows cells or specific cell populations to be marked and tracked during embryonic development.

 

Conventional Available

Chameleon (Cytbow)

Further information

Reporter line. Ubiquitous expression of nuclear-blueFP, replaced after CRE-mediated recombination by expression of eYFP or tdTomato or mCerulean.

 

Conventional

Available

Limited supply

Flamingo (TdTomato)

Further information

 

Reporter line. Ubiquitous cytoplasmic expression of tdTomato.

Conventional Available

CSF1R reporter lines

Further information

Macrophage reporter lines – CSF1R-eGFP (MacGreen) and CSF1R-mApple (MacRed).

Demonstrated utility for investigation of immune response in avians.

Used to visualise cells of the mononuclear phagocyte system (MPS; monocytes, macrophages and dendritic cells).

 

Conventional

Available

Limited supply of MacRed

Notch reporter

Further information

 

Reporter line used for real-time visualisation of Notch signalling.  Conventional

Available

 

     

Natural mutation line

Natural mutation lines Description  Facility Availability  

talpid     

Further information                                                       

Talpid mutants are classical recessive embryonic lethal chicken mutants with abnormal limb patterning and malformations.                                                                                                               

 

Conventional Available                              

Please let us know if you want further information on any of these lines or are interested in a collaboration based on our expertise

Help us to better understand how you are using eggs, chickens, and our facilities. We welcome feedback from collaborators and new users and use it to guide our research and development of new chicken lines.

Contact us