Dr Xavier Donadeu (LicVet, MS, PhD, FRS)

Reader

Address

Street

The Roslin Institute
Easter Bush Campus
Midlothian

City
Post code
EH25 9RG

Availability

  • Willingness to take Ph.D. students: Yes
    Reproductive and Developmental Biology, Veterinary Stem Cells

Background

Xavier Donadeu obtained a degree in Veterinary Medicine from Universitat Autonoma de Barcelona in 1993 followed by a brief period of clinical practice in Spain.

He then did an internship in The School of Veterinary Medicine, Louisiana State University, from where he also got a Master in Science degree in Animal Science in 1997. He completed PhD studies within the Endocrinology and Reproductive Physiology at the University of Wisconsin in 2002 followed by post-doctoral work at the Dept of Pharmacology of the Carver College of Medicine, University of Iowa.

In 2005 he moved to Edinburgh to take up a Lecturer position in the R(D)SVS of the University of Edinburgh and since 2007 he has also been a Group Leader at the Roslin Institute.

Area of Expertise

Research expertise: Animal Science, Reproductive Biology, Stem Cell Biology

 

 

Qualifications

2002 - PhD - University of Wisconsin-Madison

1997 - Master in Science - Louisiana State University

Professional Qualifications

1993 - Licentiate in Veterinary Medicine - Universitat Autonoma de Barcelona, Spain

Responsibilities & affiliations

Editor-in-chief, Domestic Animal Endocrinology

Academic Editor, PLoS One

Associate Editor, Frontiers in Veterinary Medicine

Scientific Advisory Board, Vetsina Animal Health Ltd.

The Euan MacDonald Centre

 

Undergraduate teaching

Veterinary curriculum teaching (present and past):

  • Course organiser,  Animal Body 3
  • Section organiser:  AB3 Reproduction,  Graduate Entry Programme Reproduction, AB3 Cardiovascular System,  AB3 Respiratory System
  • Animal Body 3 -  Animal Reproduction
  • Animal Body 3 (Graduate Entry Program)-   Animal Reproduction
  • Animal Body 4 
  • Clinical foundation course - Reproductive pharmacology
  • Final year clinical rotations  - Dairy cattle reproduction
  • Five-week Anatomy course for Graduate Entry Program students  

Postgraduate teaching

  • Animal Bioscience MSc (Roslin Institute)
  • Equine Science MSc (RDSVS)
  • Reproductive Biology Honours (Biological Sciences)
  • Literature awareness course (Roslin Institute)

Open to PhD supervision enquiries?

Yes

Current PhD students supervised

PhD:

  • Beth Appleby
  • Julia Falk
  • Tom Thrower
  • Madison Macleay
  • Susan Dan-Jumbo
  • Yennifer Cortes Araya
  • Tom Watson

Past PhD students supervised

PhD:

  • Ben Moore, 09/2015 to 08/2018
  • Elisabeth Rink, 03/2014 to 06/1018
  • Jason Ioannidis, 11/2012 to 09/2016
  • Bushra Mohammed, 07/2012 to 09/2016
  • Sadanand Sontakke, 10/2010 to 02/2014
  • Ruchi Sharma, 09/2010 to 09/2013
  • Stephanie Schauer, 09/2008 to 09/2012
  • Lynsey Doyle, 10/2005 to 08/2008
  • Claire Stenhouse, 09/2014 to 06/2018
  • Rebeca Martinez (Spanish visiting student), 07/2013 to 09/2013
  • Diego Cravero (Italian visiting student), 01/2012 to 07/2012 
  • Rosanna Marino (Italian visiting student), 04/2011 to 09/2011
  • Somy Nadaf (French visiting student), 12/2008 to 03/2010
  • Fernanda Goncalves (Brazilian visiting student), 05/2008 to 04/2009
  • Salina Saddick, 02/2006 to 11/2007
  • Sharron Oggle, 09/2005 to 10/2009
  • Charlotta Oddsdottir, 08/2005 to 08/2008

Research summary

I lead a research group working in the areas of Stem Cell biology and Reproductive Biology in large animal species. We are interested in these species both from a veterinary perspective and as disease models.  Work in my laboratory spans from hypothesis-led studies to understand basic cell, organ and whole animal biology and physiology, all the way through to translational studies with industry to develop novel molecular diagnostics and cell-based therapies to address specific key challenges faced by the livestock (cattle) industry and companion animal sectors. My laboratory is best known for pioneering work on 1) the use of miRNAs as novel diagnostic biomarkers for cattle, and 2) the generation and application of stem cells, particularly induced pluripotent stem cells (iPSCs), in farm species (horse, cattle, pigs). 

Current research interests

- RNA biomarkers of tissue function and disease, and novel RNA-based diagnostics for livestock - Generation of transgene-free iPSCs from large animal species (equine, bovine) and their application in Animal Health and Production - Skeletal muscle development and muscle stem cell biology in livestock species   

Knowledge exchange

Selected research contributions from my laboratory are;

- Discovery of early pregnancy biomarkers (miRNAs) in cattle (Donadeu and Ioannidis, BMC, 2016; PLoS One, 2018)

 - First comprehensive description of genome-wide profiles of small RNAs in bio-fluids in a farm animal species (Ioannidis and Donadeu, BMC, 2016), together with the first reference protocols for isolation, sequencing and validation of such RNAs (Ioannidis et al, Methods Mol Biol, 2018). 

- Discovery of miR-96/miR-183/miR-182 as a conserved miRNA cluster involved in luteal maintenance and fertility in both cattle and humans (Mohammed et al., J Clin Endo Metab 2019).

- Generation of the first ever bona fide equine iPSCs, followed by demonstration that equine and bovine iPSCs can successfully generate several health and production-relevant tissues including functional neurons (Sharma et al., 2016), mammary gland (Cravero et al., 2015) and skeletal muscle (Amilon et al., 2019).  

- First Isolation and culture of native mesenchymal stem cells (MSCs) from a domestic species, the horse (Esteves et al., Stem Cells Res Ther 2017).

- Demonstration of the therapeutic potential of endometrial MSCs in a veterinary species, the horse (Rink et al., Stem Cells Res Ther, 2018, Donadeu senior author).

 

Project activity

  • Development of novel RNA-based diagnostics for early pregnancy in cattle  (industrial partnership) 
  •  Novel iPSC-based therapies for horses (industrial partnership)
  • Antimicrobial properties of canine MSCs
  • Identifying genetic regulators of muscle development in livestock using novel stem cell technologies

Current project grants

• 2020-2023, BBSRC, Development of a high-throughput pipeline to identify causal variants and its demonstration in pig muscle’, £918K. Co-I
• 2019-2021, BBSRC Super Follow-on-Fund, ‘Translating miRNA biomarker discovery into novel veterinary diagnostics to increase the efficiency of milk production’, £500K. PI
• 2019-2023, Industrial project, ‘Stem cells for off-the-shelf applications in veterinary regenerative medicine’, £320K. PI
• 2020-2023, Dogs Trust, ‘Mesenchymal Stem/Stromal cells (MSCs) as novel tools for combating antimicrobial resistance in dogs’ £100K. Co-PI
• 2020-2021, BBSRC IAA award. ‘Novel tools for early diagnosis of mastitis in cattle’, £29,982, PI

Past project grants

• 2018-2020, Horse Betting Levy Board, ‘Axonal transport and survival in equine recurrent laryngeal neuropathy’, £230K. Co-I
• 2014-2017, Horse Betting Levy Board, ‘Exploring new sources of mesenchymal stem cells in horses’, £250K. PI
• 2012-2016, BBSRC/Zoetis, ‘miRNA biomarkers of reproductive function in cattle’, £145K. PI
• 2011-2012, Pet Plan Charitable Trust, ‘Reprogramming of keratinocytes to pluripotent stem cells for regenerative therapy in horses’, £100K. PI
• 2008-2011, Horse Betting Levy Board, ‘Derivation of pluripotent stem cells from equine fibroblasts’, £236K. PI
• 2016-2017, Scotland’s Rural College, ‘MicroRNAs as predictive biomarkers of survival and performance in dairy cattle’, £78,707. Co-PI
• 2018-2019, ISCF Transforming Food Production Seeding Award, £26,000. PI
• 2018-2019, BBSRC Pathfinder award– ‘Towards miRNA diagnostics for dairy cattle’, £12,000. PI
• 2018, Flexible Talent Mobility Award, ‘Establishing links towards implantation of miRNA-based diagnostic for livestock in Brazil’, £3,000. PI
• 2017-2018, Petplan Charitable Trust, ‘Characterising the responses of stem cells to inflammatory and infectious stimuli - towards cell-free regenerative therapies for horses’, £9,980. Co-I
• 2016-2017, Horse Betting Levy Board, ‘Exploring the potential of equine Mesenchymal Stem/Stromal Cells as antibacterial agents’, £9,931. Co-I
• 2015, Fiona and Ian Russell Seed Corn Grants, ‘Characterisation of in vivo- and in vitro-produced mesenchymal stem cells (MSCs) from horses’, £1,000. PI
• 2012-2014, CEU-Santander, ‘The luteinized unruptured follicle (LUF) in the mare: Etiopathogenic mechanisms and possible treatments to restore ovulation’, €10,000. Co-I
• 2010-2011, RCVS Trust, ‘Characterisation of horse bone marrow-derived mesenchymal stem cells and induction of their differentiation into tenocytes’, £5,000. Co-I
• 2008-2010, Thoroughbred Breeding Association, ‘Ability of exogenous LH to promote the development of ovulation-competent follicles during the transitional period in mares’, £62,082. PI
• 2008-2010, RCVS Trust, ‘Towards the generation of equine iPS cells’, £19,500. PI

View all 75 publications on Research Explorer