Publications
Publications from the lab.
2022
48. Leontiou, I., Davies, T., Clark, I., Aktar, K., Suresh, A. P., Abad, M. A., Spanos, C., Lee, K-T., Bahn, Y-S., Jeyaprakash, A. A and Hardwick, K. G. Bub1 Kinase Acts as a Signalling Hub for the Entire Cryptococcus Neoformans Spindle Assembly Checkpoint Pathway. bioRxiv https://www.biorxiv.org/content/10.1101/2022.09.21.508923v1
47. Staneva, D. P., Bresson, S., Auchynnikava, T., Spanos, C., Rappsilber, J., Jeyaprakash, A. A., Tollervey, D., Matthews, K. R and Allshire, R. C. The SPARC Complex Defines RNAPII Promoters in Trypanosoma brucei eLife (2022) in press https://doi.org/10.1101/2022.07.14.499694.
46. Kucharski, T. J., Hards, R., Vandal, S. E., Abad, M. A., Jeyaprakash, A. A., Kaye, E., Al-Rawi, A., Ly, T., Godek, K. M., Garber, S. A and Compton, D. A. Small Changes in Phospho-Occupancy at the Kinetochore-Microtubule Interface Drive Mitotic Fidelity. J. Cell Biol (2022) 221(9):e202107107 https://doi.org/10.1083/jcb.202107107
45. Abad MA*, Gupta T*, Hadders MA, Meppelink A, Wopken JP, Blackburn E, Zou J, Gireesh A, Buzuk L, Kelly DA, McHugh T, Rappsilber J, Lens SMA and Jeyaprakash AA. Mechanistic Basis for Sgo1-Mediated Centromere Localisation and Function of the CPC (2022) J Cell Biol 221 (9):e202107107 https://doi.org/10.1083/jcb.202108156 (*equal contribution)
44. Vit G, Duro J, Rajendraprasad G, Hertz EPT, Katrine L, Holland K, Weisser MB, McEwan BC, Lopez-Mendez B, Sotelo-Parrilla P, Jeyaprakash AA, Montoya G, Mailand N, Maeda K, Kettenbach A, Barisic M and Nilsson J. Chemogenetic Profiling Reveals PP2A-Independent Cytotoxicity of Proposed PP2A Activators iHAP1 and DT-061. EMBO J (2022) 41(14):e110611 https://doi.org/10.15252/embj.2022110611
43. Sankaranarayanan SR, Polisetty S, Dumbrepatil A, Jeyaprakash AA and Sanyal K. Regional Centromeres Protect Organisms From Lethal Mutations. bioRxiv (2022) Doi: https://www.biorxiv.org/content/10.1101/2022.02.25.481917v1
2021
42. Thamkachy R*, Medina-Pritchard B*, Park SO*, Chiodi CG, Zou J, de la Torre-Barranco M, Shimanaka K, Abad MA, Páramo G, Feederle R, Ruksenaite E, Heun P, Davies OR, Rappsilber J, Schneidman-Duhovny D, Cho U-S and Jeyaprakash AA. Structural Basis for Mis18 Complex Assembly: Implications for Centromere Maintenance. bioRxiv (2021) Doi:https://doi.org/10.1101/2021.11.08.466737v1 (*equal contribution)
41. Staneva DP, Carloni R, Auchynnikava T, Tong P, Jeyaprakash AA, Rappsilber J, Matthews KR and Allshire RC. A systematic analysis of Trypanosoma brucei chromatin factors identifies novel protein interaction netwoks associated with sites of transcription initiation and termination. Genome Res (2021) Doi: https://doi.org/10.1101/gr.275368.121
40. Aguilera C, Hümmer S, Masanas M, Gabau E, Guitart M, Jeyaprakash AA, Segura MR, Santamaria A and Ruiz, A. The Novel KIF1A Missesence Variant (R169R) Strongly Reduces Microtubule Stimulated ATPase Activity and Is Associated With NESCAV Syndrome. Frontiers in Neuroscience (2021) 15:618098. Doi: https://doi.org/10.3389/fnins.2021.618098
39. Su BX, Wang M, Schaffner C, Nerusheva OO, Clift D, Spanos C, Wallek A, Wu Y, Wu, Y, Rappsilber J, Jeyaprakash AA, Storchova Z, Hay R and Marsten AL. Sumoylation stabilizes Stabilizes Sister Kinetochore Biorientation to Allow Timely Anaphase. J. Cell Biol (2021) Jul 5; 220(7):e202005130. Doi: https://doi.org/10.1083/jcb.202005130.
38. Ueki Y, Hadders M, Weisser MB, Nasa I, Sotelo-Parrilla P, Cressey LE, Gupta T, Hertz ET, Kruse T, Montoya G, Jeyaprakash AA, Kettenbach A, Lens SMA and Nilsson J (2021) A highly conserved binding pocket on PP2A-B56 is required for hSgo1 binding and cohesion protection during mitosis. EMBO Rep (2021) May11;e52295. Doi: https://doi.org/10.15252/embr.202052295
2020
37. Pesenti E, Liskovykh M, Okazaki K, Mallozzi A, Reid C, Abad MA, Jeyaprakash AA, Kouprina N, Larionov V, Matsumoto H and Earnshaw W (2020) Analysis of Complex DNA Rearrangements During Early Stages of HAC formation. ACS Synth. Biol. 9, 12, 3267-3287.
36. Ambrose AJ, Pham NT, Sivinski J, Guimarães L, Mollasalehi N, Jimenez P, Abad MA, Jeyaprakash AA, Shave S, Costa-Lotufo L, La Clair JJ, Auer M and Chapman (2020) A two-step resin based approach to reveal survivin-selective fluorescent probes. RSC Chemical Biology. DOI:10.1039/d0cb00122h
35. Wimbish RT, DeLuca KF, Mick JE, Himes J, Sanchez IJ, Jeyaprakash AA and DeLuca JG (2019) The Hec1/Ndc80 tail domain is required for force generation at kinetochores, but is dispensable for kinetochore-microtubule attachment formation and Ska complex recruitment. Mol Biol Cell. DOI: 10.1091/mbc.E20-05-0286
34. Medina-Pritchard B, Lazou V, Zou J, Byron O, Maria A Abad, Rappsilber J, Heun P and Jeyaprakash AA (2020). Structural Basis for Centromere Maintenance by Drosophila CENP-A chaperone Cal1. EMBO J. DOI: 10.15252/embj.2019103234
2019
33. Roure V, Medina-Pritchard B, Anselm E, Jeyaprakash AA and Heun, P (2019) Reconstituting Drosophila Centromere Identity in Human Cells. Cell Rep. DOI: 10.1016/j.celrep.2019.08.067
32. Abad MA, Ruppert JG*, Buzuk L*, Wear M, Zou J, Webb KM, Kelly DA, Voigt P, Rappsilber J, Earnshaw WC and Jeyaprakash AA (2019) Borealin-Nucleosome Interaction Secures Chromosome Association of the Chromosomal Passenger Complex. J Cell Biol. DOI: 10.1083/jcb.201905040 (* equal contribution)
31. Leontiou I, London N, May KM, Ma Y, Grzesiak L, Medina-Pritchard B, Jeyaprakash AA, Biggins S and Hardwick KG (2019) The Bub1-TPR Domain Directly Interacts with Mad3 to Generate a Robust Spindle Checkpoint Arrest. Current Biology. DOI: 10.1016/j.cub.2019.06.11
30. Capalbo L, Mela I, Abad MA, Jeyaprakash AA, Edwarson JM and D'Avino PPRoure V (2019) Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phophorylation Assays. Methods Mol Biol. DOI: 10.1007/978-1-4939-9492-2_15
2018
29. Anselm E, Thomae AW, Jeyaprakash AA and Heun, P (2018) Oligomerization of Drosophila Nucleophasmin-like protein is required for its centromere localisation. Nucleic Acids Res DOI: 10.1093/narlgky988
28. de la Roche M, Hamilton C, Mortensen R, Jeyaprakash AA, Gosh S and Anand PK (2018) Trafficking of cholesterol to the ER is required for NLRP3 inflammasome activation. J Cell Biol. DOI: 10.1083/jcb.201709057
27. Cruz M, Abad MA, van Ijcken W, Galjart N, Jeyaprakash AA, Maiato H and Ferrás C. (2018) Mitotic progression, arrest, exit or death is determined by centromere integrity and independent of de novo transcription. eLife. DOI: 10.7554/eLife.36898
26. Ruppert JG, Samejima K, Platani M, Molina O, Kimura H, Jeyaprakash AA, Ohta S and Earnshaw WC. (2018) Heterochromatin protein 1a targets the CPC for activation at heterochromatin before mitotic entry. EMBO J. DOI: 10.15252/embj.201797677
25. Welburn J* and Jeyaprakash AA*. Mechanisms of Mitotic Kinase Regulation: A Structural Perspective. Front. Cell Dev. Biol. DOI: 10.3389/fcell.2018.00006 (*Corresponding authors)
2017
24. Spiller F*, Medina-Pritchard M*, Abad MA*, Wear MA, Molina O, Earnshaw WC and Jeyaprakash AA. Molecular Basis for Cdk1 Regulated Timing of Mis18 Complex Assembly and CENP-A Deposition. EMBO Rep. DOI: 10.15252/embr.201643564 (*equal contribution)
2016
23. Molina O, Vargiu G, Abad MA, Zhiteneva A, Jeyaprakash AA, Masumoto H, Kouprina N, Larionov V and Earnshaw WC. Epigenetic Engineering Reveals a Balance Between Histone Modifications and Transcription in Kinetochore Maintenance. Nat Commun. DOI: 10.1038/ncomms13334.
22. Capalbo L, Mela I, Abad MA, Jeyaprakash AA, Edwardson JM and D’Avino PP. The ESCRT-III Component CHMP4C is Regulated Via a Relay Mechanism During Abscission. Open Biol. DOI: 10.1098/rsob.160248
21. Abad MA, Zou J, Medina-Pritchard M, Nigg EA, Rappsilber J, Santamaria A and Jeyaprakash AA. (2016) Ska3 Ensures Timely Mitotic Progression by Interacting Directly with Microtubules and Ska1 Microtubule Binding Domain. Sci Rep. DOI: 10.1038/srep34042
20. Subramanian L*, Medina-Pritchard B*, Barton R, Spiller F, Kulasegaran-Shylini R, Radaviciute G, Allshire RC and Jeyaprakash AA. (2016) Centromere Localisation and Function of Mis18 Requires Yipee-Like Domain-Mediated Oligomerisation. EMBO Rep. DOI: 10.15252/embr.201541520 (*equal contribution)
2015
19. Papini D, Langemeyer L, Abad MA, Kerr A, Samejima I, Eyers PA, Jeyaprakash AA, Higgins JMG, Barr FA and Earnshaw WC. (2015) TD60 Links RalA GTPase Function to the CPC in Mitosis. Nat Commun. DOI: 10.1038/ncomms8678
18. Platani M, Trinkle-Mulchay L, Porter M, Jeyaprakash AA and Earnshaw WC. (2015) Mio Depletion Links mTOR Regulation to Aurora A and Plk1 Activation at Mitotic Centrosomes. J Cell Biol. DOI: 10.1083/jcb.201410001
2014
17. Abad MA*, Medina B*, Santamaria A*, Zou J, Plasberg-Hill C, Madhumalar A, Jayachandran U, Redli PM, Rappsilber J, Nigg EA and Jeyaprakash AA. (2014) Structural Basis for Microtubule Recognition by the Human Kinetochore Ska complex. Nat Commun. DOI: 10.1038/ncomms3964 (*equal contribution)
2013
16. Sharma A, Pohlentz G, Bobbili KB, Jeyaprakash AA, Chandran T, Mormann M, Swamy MJ and Vijayan M. (2013) The Sequence and Structure of Snake Gourd (Trichosanthes Anguina) Seed Lectin, a Three-Chain Nontoxic Homologue of Type II RIP. Acta Cryst. DOI: 10.1107/S0907444913010020
2012
15. Jeyaprakash AA, Santamaria A, Jayachandran U, Chan YW, Benda C, Nigg EA and Conti E. (2012) Structural and Functional Organization of the Ska Complex, a Key Component of the Kinetochore-Microtubule Interface. Mol Cell. DOI: 10.1016/j.molcel.2012.03.005
14. Chan YW, Jeyaprakash AA, Nigg EA and Santamaria A. (2012) Aurora B Controls Kinetochore-Microtubule Attachments by Inhibiting Ska Complex-KMN Network Interaction. J Cell Biol. DOI: 10.1083/jcb.201109001
2011
13. Jeyaprakash AA§, Basquin C, Jayachandran U, Conti E§. (2011) Structural Basis for the Recognition of Phosphorylated Histone H3 by the Survivin Subunit of the Chromosomal Passenger Complex. Structure. DOI: 10.1016/j.str.2011.09.002 (§Corresponding Authors)
2009
12. Gaitanos TN, Santamaria A, Jeyaprakash AA, Wang B, Conti E, Nigg EA. (2009) Stable Kinetochore-Microtubule Interactions Depend on the Ska Complex and its New Component Ska3/C13orf3. EMBO J. DOI: 10.1038/emboj.2009.96
2007
11. Jeyaprakash AA*, Klein UR*, Lindner D, Ebert J, Nigg EA, Conti E. (2007) Structure of a Survivin-Borealin-INCENP Core Complex Reveals How Chromosomal Passengers Travel Together. Cell. DOI: 10.1016/j.cell.2007.07.045 (*equal contribution) Comments in:
- Ruchaud S, Carmena M, and Earnshaw WC. (2007) The Chromosomal Passenger Complex: One for all and all for one. Cell. 131, 230-231.
- Heinrichs A. (2007) Cell Cycle Passengers travel together. Nat. Rev. Mol. Cell. Biol 8, 944.
- Max Plank Institute Press Release
2005
10. Jeyaprakash AA, Jayashree G, Mahanta SK, Swaminathan CP, Sekar K, Surolia A, Vijayan M. (2005) Structural Basis for the Energetics of Jacalin-Sugar Interactions: Promiscuity Versus Specificity. J Mol Biol. DOI: 10.1016/j.jmb.2004.03.040
2004
9. Jeyaprakash AA, Srivastav A, Surolia A, Vijayan M. (2004) Structural Basis for the Carbohydrate Specificites of Artocarpin: Variation in the Length of a Loop as a Strategy for Generating Ligand Specificity. J Mol Biol. DOI: 10.1016/j.jmb.2004.03.040
8. Natchiar SK, Jeyaprakash AA, Ramya TNC, Thomas CJ, Suguna K, Surolia A, Vijayan M. (2004) Structural Plasticity of Peanut Lectin: An X-ray Analysis Involving Variation in pH, Ligand Binding and Crystal Structure. Acta Crystallogr D Biol Crystallogr. DOI: 10.1107/S090744490302849X
2003
7. Sekar K, Mala SV, Rajakannan V, Yogavel M, Velmurugan D, Poi MJ, Vishwanath S, Gowda TV, Jeyaprakash AA, Tsai MD. (2003) Crystal Structure of the Free and Anisic Acid Bound Triple Mutant of Phospholipase A2. J Mol Biol. DOI: 10.1016/j.jmb.2003.08.032
6. Jeyaprakash AA, Katiyar S, Swaminathan CP, Sekar K, Surolia A, Vijayan M. (2003) Structural Basis of the Carbohydrate Specificities of Jacalin: An X-ray and Modeling Study. J Mol Biol. DOI: 10.1016/s0022-2836(03)00901-x
2002
5. Rajakannan V, Yogavel M, Poi MJ, Jeyaprakash AA, Jeyakanthan J, Velmurugan D, Tsai MD, Sekar K. (2002) Observation of Additional Calcium Ion in the Crystal Structure of the Triple Mutant K56,120,121M of Bovine Pancreatic Phospholipase A2. J Mol Biol. DOI: 10.1016/s0022-2836(02)01132-4
4. Jeyaprakash AA, Rani PG, Reddy GB, Banumathi S, Betzel C, Sekar K, Surolia A, Vijayan M. (2002) Crystal Structure of the Jacalin-T-antigen Complex and a Comparative Study of Lectin-T-antigen Complexes. J Mol Biol. DOI: 10.1016/s0022-2836(02)00674-5
3. Pratap JV, Jeyaprakash AA, Rani PG, Sekar K, Surolia A, Vijayan M. (2002) Crystal Structures of Artocarpin, a Moraceae Lectin with Mannose Specificity, and its Complex with Methyl-alpha-D-Mannose: Implications to the Generation of Carbohydrate Specificity. J Mol Biol. DOI: 10.1006/jmbi.2001.5432
2001
2. Adhikari P, Bachhawat-Sikder K, Thomas CJ, Ravishankar R, Jeyaprakash AA, Sharma V, Vijayan M, Surolia A. (2001) Mutational Analysis at Asn41 in Peanut Agglutinin. A Residue Critical for the Binding of the Tumor-Associated Thomsen-Friedenreich Antigen. J Biol Chem. DOI: 10.1074/jbc.M103040200
1. Manoj N, Jeyaprakash AA, Pratap JV, Komath SS, Kenoth R, Swamy KJ, Vijayan M. (2001) Crystallization and Preliminary X-ray Studies of Snake Gourd Lectin: Homology with TypeII Ribosome-Inactivating Proteins. Acta Crystallogr D Biol Crystallogr. DOI: 10.1107/s0907444901004620