Professor Stefan Westermann - Center of Medical Biotechnology, Department of Molecular Genetics, University of Duisburg-Essen
9th January 2017 at 12:00pm [Download iCalendar / .ics file]
Centromeres direct the assembly of kinetochores, microtubule-attachment sites thatallow chromosome segregation on the mitotic and meiotic spindle. I will discuss current projects in the lab that aim to elucidate the molecular organization of these chromosome segregation machines through a biochemical and genetic analysis of the budding yeast kinetochore. We have biochemically reconstituted large parts of the yeast kinetochore, including a full-length ten-protein KMN network. Current projects in the lab address how KMN is anchored to centromeric chromatin through association with multiple recruiters such as the CENP-C homolog Mif2, the AO complex and the CENP-T homolog Cnn1.In addition, we are combining biochemical reconstitution experiments with single-molecule fluorescence microscopy to analyze mechanisms of microtubule-based motility. To this end we have performed a detailed analysis of the kinesin-14 protein Kar3, a minus-end directed molecular motor involved in microtubule organization and kinetochore transport. We are asking how the unusual biophysical properties of the Kar3 motor, whose movement depends on a non-catalytic domain, contribute to microtubule organization and kinetochore function.
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