Professor Dusan Uhrin

Chair of NMR Spectroscopy

Research summary

NMR spectroscopy, NMR methodology development, complex mixtures analysis

Research Overview

New NMR Methodology

Using 60 to 800 MHz NMR spectrometers, many equipped with CryoProbesTM, we are developing liquid-state NMR methodology for diverse branches of science. In particular, we work on increasing the sensitivity of NMR spectroscopy. Recent application of our SHARPER NMR concetp9,1,2 has seen up to 30-fold sensitivity improvement of benchtop NMR.1

Complex Mixtures

The structure elucidation of compounds contained within complex mixtures is a challenging task. We study various mixtures such as those fund in the environment, living organisms or food and beverages, e.g. Scotch whisky.5,6,8 Using metabolomics approaches,4 we collaborate with colleagues in biology, agriculture and medicine.

  1. Dickson, C.L., Peat, G. Rossetto, M., Halse, M.E. and Uhrín, D., SHARPER-enhanced benchtop NMR: improving SNR by removing couplings and approaching natural linewidths, Chem. Commun., 58, 5534-5537 (2022).
  2. Davy, M., Dickson, C.L., Wei, R., Uhrín, D., Butts, C.P. Monitoring off-resonance signals with SHARPER NMR – the MR-SHARPER experiment, Analyst, 147, 1702-1708 (2022).
  3. Wei, R., Dickson, C.L., Uhrín, D., Lloyd-Jones, G.C. Rapid Estimation of T1 for Quantitative NMR J. Org. Chem. 86 9023–9029 (2021).
  4. Bica, R., Palarea-Albaladejo, J., Kew, W., Uhrín, D, Pacheco, D., Macrae, A., Dewhurst, R.J. Nuclear Magnetic Resonance to Detect Rumen Metabolites Associated with Enteric Methane Emissions from Beef Cattle, Sci Rep, 10, 5578 (2020.
  5. Stockwell, M., Goodall, I. Uhrín, D, Quantification of whisky congeners by 1H NMR spectroscopy, Anal. Sci. Adv. 1,132–140(2020).
  6. Kew, W., Goodall, I., Uhrín, D. Analysis of Scotch Whisky by 1H NMR and chemometrics yields insight into its complex chemistry, Food Chemistry. 298, 125052 (2019).
  7. Brodaczewska, N., Košťálová, Z. Uhrín, D, (3, 2)D 1H, 13C BIRDr,X-HSQC-TOCSY for NMR structure elucidation of mixtures. Application to complex carbohydrates, J Biomol. NMR 70,115–122 (2018).
  8. Kew, W., Bell, N.G.A. Goodall, I., Uhrín, D, Advanced solvent signal suppression for the acquisition of 1D and 2D NMR spectra of Scotch Whisky, Magn. Reson. Chem. 55, 785–796, (2017).
  9. Jones, A.B., Lloyd-Jones, G.C., Uhrín, D, SHARPER reaction monitoring: generation of a narrow line-width NMR singlet, without X-pulses, in an inhomogeneous magnetic field, Anal. Chem. 89 10013–10021 (2017).