Keisuke Kaji
Address
- Street
-
Centre for Regenerative Medicine,
Institute for Regeneration and Repair,
The University of Edinburgh,
Edinburgh BioQuarter,
5 Little France Drive, - City
- Edinburgh
- Post code
- EH16 4UU
Background
- 2016- Professor of Biology of Reprogramming
- 2016- MRC Senior Non-Clinical Fellow
- 2008- Group Leader, MRC Centre for Regenerative Medicine, University of Edinburgh
- 2003-2007 Postdoctral fellow in Dr. Brian Hendrich group at Institute for Stem Cell Research, The University of Edinburgh
- 2003 PhD Department of Biological Information, Tokyo Institute of Technology
- 2000 MSc Department of Life Science, Tokyo Institute of Technology
- 1998 BSc Department of Life Science, Tokyo Institute of Technology
Research summary
Biology of reprogramming
The Kaji group aims to understand the mechanisms of cellular reprogramming, how cellular identify can be faithfully controlled by exogenous gene expression manipulation, in order to improve the technology and to generate useful cell types for medicine.
Aims and areas of interest
Recently a technology to generate pluripotent stem cells from differentiated somatic cells has been developed using defined transcription factors. Similarly, different cellular identity conversions from one type to the other have also been achieved by exogenous expression of master transcription factors. However, the biological mechanism underlying the process of reprogramming has not been elucidated well, and the resulting cell types are often not fully functional. Our group aims to understand the mechanism of the reprogramming, improve the technology and generate fully functional cell types for medicine.
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B1 SINE-binding ZFP266 impedes mouse iPSC generation through suppression of chromatin opening mediated by reprogramming factors
In:
Nature Communications
DOI: https://doi.org/10.1038/s41467-023-36097-9
Research output: Contribution to Journal › Article (Published) -
Conserved regulation of RNA processing in somatic cell reprogramming
In:
BMC Genomics, vol. 20
DOI: https://doi.org/10.1186/s12864-019-5438-2
Research output: Contribution to Journal › Article (Published) -
Fine-Tuning Mybl2 Is Required for Proper Mesenchymal-to-Epithelial Transition during Somatic Reprogramming
In:
Cell Reports, vol. 24, pp. 1496-1511.e8
DOI: https://doi.org/10.1016/j.celrep.2018.07.026
Research output: Contribution to Journal › Article (Published) -
Mapping transcription factor occupancy using minimal numbers of cells in vitro and in vivo
(15 pages)
In:
Genome Research, vol. 28, pp. 592-605
DOI: https://doi.org/10.1101/gr.227124.117
Research output: Contribution to Journal › Article (Published) -
Constitutively active Smad2/3 are broad scope potentiators of transcription factor-mediated cellular reprogramming
(24 pages)
In:
Cell Stem Cell, vol. 21, pp. 791-805.e9
DOI: https://doi.org/10.1016/j.stem.2017.10.013
Research output: Contribution to Journal › Article (Published) -
Coupling shRNA screens with single-cell RNA-seq identifies a dual role for mTOR in reprogramming-induced senescence
(14 pages)
In:
Genes and Development, vol. 31, pp. 2085-2098
DOI: https://doi.org/10.1101/gad.297796.117
Research output: Contribution to Journal › Article (Published) -
Reprogramming roadblocks are system-dependent
In:
Stem Cell Reports, vol. 5, pp. 350–364
DOI: https://doi.org/10.1016/j.stemcr.2015.07.007
Research output: Contribution to Journal › Article (Published) -
Acute Loss of Cited2 Impairs Nanog Expression and Decreases Self-Renewal of Mouse Embryonic Stem Cells
(14 pages)
In:
STEM CELLS, vol. 33, pp. 699-712
DOI: https://doi.org/10.1002/stem.1889
Research output: Contribution to Journal › Article (Published) -
piggyBac Transposon Mediated Reprogramming and Flow Cytometry Analysis of CD44 and ICAM1 Cell-Surface Marker Changes
DOI: https://doi.org/10.1007/7651_2014_147
Research output: › Chapter (Published) -
Novel tools to discern the pluripotency gene network establishment
(1 page)
In:
Transgenic Research, vol. 23, pp. 848-848
Research output: Contribution to Journal › Meeting abstract (Published) -
Routes to induced pluripotent stem cells
(5 pages)
In:
Current Opinion in Genetics and Development, vol. 28, pp. 38-42
DOI: https://doi.org/10.1016/j.gde.2014.08.006
Research output: Contribution to Journal › Literature review (Published) -
MBD3/NuRD Facilitates Induction of Pluripotency in a Context-Dependent Manner
(9 pages)
In:
Cell Stem Cell, vol. 15, pp. 102-110
DOI: https://doi.org/10.1016/j.stem.2014.04.019
Research output: Contribution to Journal › Article (Published) -
High-resolution analysis with novel cell-surface markers identifies routes to iPS cells
(4 pages)
In:
Nature, vol. 499, pp. 88-91
DOI: https://doi.org/10.1038/nature12243
Research output: Contribution to Journal › Article (Published) -
ERK2 Suppresses Self-Renewal Capacity of Embryonic Stem Cells, but Is Not Required for Multi-Lineage Commitment
In:
PLoS ONE, vol. 8
DOI: https://doi.org/10.1371/journal.pone.0060907
Research output: Contribution to Journal › Article (Published) -
Transcriptional Activation by Oct4 Is Sufficient for the Maintenance and Induction of Pluripotency
(11 pages)
In:
Cell Reports, vol. 1, pp. 99-109
DOI: https://doi.org/10.1016/j.celrep.2011.12.002
Research output: Contribution to Journal › Article (Published) -
New strategies to generate induced pluripotent stem cells
(6 pages)
In:
Current opinion in biotechnology, vol. 20, pp. 516-521
DOI: https://doi.org/10.1016/j.copbio.2009.09.005
Research output: Contribution to Journal › Literature review (Published) -
piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells
(5 pages)
In:
Nature, vol. 458, pp. 766-770
DOI: https://doi.org/10.1038/nature07863
Research output: Contribution to Journal › Article (Published) -
Virus-free induction of pluripotency and subsequent excision of reprogramming factors
(6 pages)
In:
Nature, vol. 458, pp. 771-775
DOI: https://doi.org/10.1038/nature07864
Research output: Contribution to Journal › Article (Published) -
Mbd3, a component of the NuRD co-repressor complex, is required for development of pluripotent cells
(10 pages)
In:
Development, vol. 134, pp. 1123-32
DOI: https://doi.org/10.1242/dev.02802
Research output: Contribution to Journal › Article (Published) -
The NuRD component Mbd3 is required for pluripotency of embryonic stem cells
(8 pages)
In:
Nature Cell Biology, vol. 8, pp. 285-92
DOI: https://doi.org/10.1038/ncb1372
Research output: Contribution to Journal › Article (Published)
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Abdenour Soufi
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Donal O'Carroll
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Tilo Kunath
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Stuart Forbes
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David Hay
- Jesus Gil, MRC Clinical Sciences Centre, UK
- Kosuke Yusa, Kyoto University, Japan
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Douglas Strathdee, Beatson Institute, UK