Thesis title: Estrogenic Profiling in Lung in Pulmonary Arterial Hypertension by Mass Spectrometry Imaging
MRC Precision Medicine
Year of study: 3
- Centre for Cardiovascular Science
- College of Medicine and Veterinary Medicine
- Email: email@example.com
Centre for Cardiovascular Science
The Queen's Medical Research Institute
University of Edinburgh
47 Little France Crescent
- Post code
- EH16 4TJ
2014-2019: Chemistry with a Year Abroad, MChem (Hons); University of Edinburgh
The primary aims of James' PhD research include:
- In-depth examination of estrogen metabolism in human primary cell models of pulmonary arterial hypertension (PAH) to allow for a comparative study off the effects on the sexes, as well as an assesment of the similarities and differences between the various forms of the disease (genetic, idiopathic) using LC-MS/MS.
Regional surface profiling of estrogens in cross-sectional lung tissue samples from rodent models of PAH using MS imaging, coupled with the development of a protocol for the derivatisaiton and chemical tagging of estrogenic metabolites on the tissue surface.
Further development of MS imaging to provide an insight into wider lipidomic changes in lung tissue affected by PAH, such as oxidative stress. The use of spatial lipidomics, will allow for the evaluation and assessment of changes in rodent model lung .