Thesis title: Estrogenic Profiling in Lung in Pulmonary Arterial Hypertension by Mass Spectrometry Imaging
2014-2019: Chemistry with a Year Abroad, MChem (Hons); University of Edinburgh
The primary aims of James' PhD research include:
- In-depth examination of estrogen metabolism in human primary cell models of pulmonary arterial hypertension (PAH) to allow for a comparative study off the effects on the sexes, as well as an assesment of the similarities and differences between the various forms of the disease (genetic, idiopathic) using LC-MS/MS.
Regional surface profiling of estrogens in cross-sectional lung tissue samples from rodent models of PAH using MS imaging, coupled with the development of a protocol for the derivatisaiton and chemical tagging of estrogenic metabolites on the tissue surface.
Further development of MS imaging to provide an insight into wider lipidomic changes in lung tissue affected by PAH, such as oxidative stress. The use of spatial lipidomics, will allow for the evaluation and assessment of changes in rodent model lung .