Dr Joanna Sadler
BBSRC Discovery Fellow in Biotechnology

Contact details
- Email: joanna.sadler@ed.ac.uk
Background
(2022 Chancellor's Fellow, University of Edinburgh)
2019-2022 BBSRC Discovery Fellow, University of Edinburgh
2018 Postdoctoral Research Assistant, University of St Andrews
2017 Postdoctoral Research Assistant, Manchester Institute of Biotechnology, University of Manchester
Qualifications
2017 D. Phil, Organic Chemistry and Biocatalysis, University of Strathclyde/GlaxoSmithKline Collaborative PhD Programme
2013 MSci, Chemistry with a Year in Industry, University of Bristol
Responsibilities & affiliations
Founder and Director of Leaders in Science (www.leadersinscience.co.uk)
EUSynBioS Steering Committee Member
Research summary
Plastic degradation and up-cycling * Microbial pathway engineering * Sustainable chemical synthesis * Biocompatible chemistry * Waste valorisation * Directed evolution and adaptive evolution * Biocatalysis
Current research interests
Plastics are deeply embedded in modern day society, yet their current lifecycle is unsustainable. These recalcitrant materials are derived from finite, fossil fuel based resources and are largely used for single-use products which contribute to the plastic pollution crisis. I am interested in using synthetic biology blended with synthetic chemistry to develop industrially relevant methods to valorise plastic waste into high value small molecules. Not only will this remove waste plastic from circulation, but will enables sustainable routes to bulk and fine chemicals which are otherwise derived directly from petrochemical feedstocks. My research in this field can be broadly broken down into three main objectives: (1) novel plastic up-cycling pathways, (2) biocompatible plastic degradation technologies, (3) using plastic waste as a feedstock for microbial growth.-
Overproduction of native and click-able colanic acid slime from engineered escherichia coli
(6 pages)
In:
JACS Au, vol. 3, pp. 378-383
DOI: https://doi.org/10.1021/jacsau.2c00583
Research output: Contribution to Journal › Article (Published) -
Tyramine derivatives catalyze the aldol dimerization of butyraldehyde in the presence of E. coli
(7 pages)
In:
ChemBioChem, vol. 23
DOI: https://doi.org/10.1002/cbic.202200238
Research output: Contribution to Journal › Article (Published) -
SpeedyGenesXL: An automated, high-throughput platform for the preparation of bespoke ultralarge variant libraries for directed evolution
(17 pages)
DOI: https://doi.org/10.1007/978-1-0716-2152-3_5
Research output: › Chapter (E-pub ahead of print) -
Bacteria serve up a tasty solution to the global plastic problem
(5 pages)
In:
The Biochemist, vol. 43, pp. 28-32
DOI: https://doi.org/10.1042/BIO_2021_188
Research output: Contribution to Journal › Article (Published) -
Microbial synthesis of vanillin from waste poly(ethylene terephthalate)
(8 pages)
In:
Green Chemistry, vol. 23, pp. 4665-4672
DOI: https://doi.org/10.1039/D1GC00931A
Research output: Contribution to Journal › Article (E-pub ahead of print) -
Interfacing non-enzymatic catalysis with living microorganisms
(11 pages)
In:
RSC Chemical Biology, vol. 2, pp. 1073-1083
DOI: https://doi.org/10.1039/d1cb00072a
Research output: Contribution to Journal › Review article (E-pub ahead of print) -
SynBio: Green and clean sustainable solutions for designer pharmaceuticals, catalysis, and bioremediation
In:
Current opinion in chemical biology, vol. 58, pp. A1-A3
DOI: https://doi.org/10.1016/j.cbpa.2020.11.001
Research output: Contribution to Journal › Editorial (E-pub ahead of print) -
The bipartisan future of synthetic chemistry and synthetic biology
In:
ChemBioChem
DOI: https://doi.org/10.1002/cbic.202000418
Research output: Contribution to Journal › Comment/debate (Published) -
S-Adenosyl methionine cofactor modifications enhance the biocatalytic repertoire of small molecule C-Alkylation
In:
Angewandte Chemie International Edition, vol. 58, pp. 17583-17588
DOI: https://doi.org/10.1002/anie.v58.49
Research output: Contribution to Journal › Article (E-pub ahead of print) -
Phenylalanine meta‐hydroxylase: A single residue mediates mechanistic control of aromatic amino acid hydroxylation
In:
ChemBioChem, vol. 21
DOI: https://doi.org/10.1002/cbic.201900320
Research output: Contribution to Journal › Article (E-pub ahead of print) -
GeneORator: An effective strategy for navigating protein sequence space more efficiently through boolean or-type DNA libraries
In:
ACS Synthetic Biology, vol. 8, pp. 1371-1378
DOI: https://doi.org/10.1021/acssynbio.9b00063
Research output: Contribution to Journal › Article (Published) -
Ultra-high throughput functional enrichment of large monoamine oxidase (MAO-N) libraries by fluorescence activated cell sorting
In:
Analyst, vol. 143, pp. 4747-4755
DOI: https://doi.org/10.1039/C8AN00851E
Research output: Contribution to Journal › Article (E-pub ahead of print) -
Fast and flexible synthesis of combinatorial libraries for directed evolution
(21 pages)
DOI: https://doi.org/10.1016/bs.mie.2018.04.006
Research output: › Chapter (E-pub ahead of print) -
A tandem enzymatic sp2-C-methylation process: Coupling in situ s-adenosyl-L-methionine formation with methyl transfer
In:
ChemBioChem, vol. 18, pp. 992-995
DOI: https://doi.org/10.1002/cbic.201700115
Research output: Contribution to Journal › Article (Published) -
Structural and functional basis of C-Methylation of coumarin scaffolds by NovO
In:
Acs chemical biology, vol. 12, pp. 374-379
DOI: https://doi.org/10.1021/acschembio.6b01053
Research output: Contribution to Journal › Article (Published) -
Imidazolium-tagged glycan probes for non-covalent labeling of live cells
In:
Chemical Communications, vol. 52, pp. 4906-4909
DOI: https://doi.org/10.1039/C5CC10040B
Research output: Contribution to Journal › Article (Published) -
Chemoselective sequential click ligations directed by enhanced reactivity of an aromatic ynamine
In:
Organic letters, vol. 18, pp. 1694-1697
DOI: https://doi.org/10.1021/acs.orglett.6b00635
Research output: Contribution to Journal › Article (Published)