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Building a model transcriptome for ruminants: the sheep gene expression atlas project and beyond

Seminar by Dr Emily Clark, The Roslin Institute.

Abstract

Small ruminants are an important source of meat, milk and fibre for the global livestock sector. To support functional annotation of the sheep genome (currently, Oar v4) we have produced a high-resolution atlas of gene expression from a comprehensive set of sheep tissues and cell types. This work was published in PLoS Genetics in 2017, providing a model transcriptome for ruminants. Since then we have produced several satellite manuscripts describing the non-coding transcriptome, methodology for merging multiple RNA-Seq datasets and a comprehensive profiling of transcription during the development of the ruminant GI tract. We are currently building on the sheep gene expression atlas by describing allele-specific expression, across tissues and cell types and during early foetal development. To apply these concepts to sheep with varying genetic backgrounds, we have been sequencing the genomes of hundreds of African sheep and goats, within the Centre for Tropical Livestock Genetics and Health. I will describe these projects and our wider vision for how we can use functional genomics to identify variants that are relevant for breeding for inclusion in genomic prediction models, and find loci that are involved in adaptation to a tropical environment. Then finally I will give a brief summary of how generating large-scale functional genomics datasets from livestock can help us to harness functional variation and turn it into sustainable genetic gain.

Apr 03 2019 -

Building a model transcriptome for ruminants: the sheep gene expression atlas project and beyond

Seminar by Dr Emily Clark, The Roslin Institute.

Roslin Institute auditorium