RUNX1 reporter lines
RUNX1-eGFP and RUNX1-Cre Cytbow
Summary & Utility
The RUNX1-eGFP line is a transgenic reporter chicken line with Runt-related transcription factor 1 (RUNX1) promoter/enhancer driven eGFP expression and is based upon the P1 promoter and the +23 enhancer1 of the chicken RUNX1 locus. The RUNX1-eGFP line enables the visualisation of chicken haematopoietic cells and haematopoietic cell progenitor populations2.
Similar to the CSF1R reporter lines the RUNX1-eGFP line can be used to visualise chicken lymphoid tissues by whole mount fluorescence microscopy (Figure 1). In conjunction with the CSF2R staining3, the RUNX1-eGFP line can also be used to simultaneously visualise and distinguish avian granulocytes (Basophils, Eosinophils and Heterophils) and monocytes by flow cytometry (Figure 2). The degranulation of heterophils can also be quantified.
RUNX1-Cre Cytbow line
The RUNX1-Cre Cytbow line is a transgenic reporter chicken line with chicken RUNX1 promoter/enhancer driven Cre recombinase expression. The RUNX1-Cre Cytbow line uses the same promoter/enhancer as the RUNX1-eGFP line to drive Cre recombinase expression. This line is the product of a cross between a RUNX1-Cre line and the Chameleon line. The RUNX1-Cre Cytbow line enables cell lineage tracing and clonal analysis of selected cell populations, such as haematopoietic cells post-hatch, skeletal muscle cells and epithelial cells (Figure 3). Fertile eggs produced from this line have both RUNX1-Cre and Cytbow (double transgenic).
The generation of the RUNX1-eGFP and RUNX1-Cre Cytbow lines was funded by the Medical Research Council (MR/L018160/1). These RUNX1 lines were created by the use of lentiviral gene transfer. The RUNX1 control elements utilised in the construction of the transgene consist of the P1 promoter and the +23 enhancer1. The cell lineage specificity of reporter gene expression was initially confirmed by transfecting chick embryonic blood cells with the RUNX1-eGFP construct to report Runt-related transcription factor 1 gene activity. These lines were developed by Dr Adam Balic and Professor Helen Sang.
To reference this line for publications please contact Dr Adam Balic.
- Bee, T., Ashley, E. L., Bickley, S. R., Jarratt, A., Li, P. S., Sloane-Stanley, J., Göttgens, B. & de Bruijn, M. F. The mouse Runx1 +23 hematopoietic stem cell enhancer confers hematopoietic specificity to both Runx1 promoters. Blood. 113(21):5121-4 (2009).
- Davey, M. G., Balic, A., Rainger, J., Sang, H. M. & McGrew, M. J. Illuminating the chicken model through genetic modification. Int. J. Dev. Biol. 62, 257–264 (2018).
Wu, Z., Hu, T., Chintoan-Uta, C., Macdonald, J., Stevens, M. P., Sang, H., et al. Development of novel reagents to chicken FLT3, XCR1 and CSF2R for the identification and characterization of avian conventional dendritic cells. Immunology 165, 171–194. (2022).