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Multiphoton (2-photon) Microscopy

The Multiphoton Intravital Microscope is located within Lab G.069.

Multiphoton Microscopy sees deeper into live tissue (incubation for in vitro slices also available) and is less phototoxic than pinhole-based confocal microscopy. To make use of this technology, contact Bob Fleming in the first instance.

 

  • Zeiss LSM7MP upright Multiphoton Microscope.
  • Coherent Chamaeleon Ultra II Ti:Sa tunable laser with OPO wavelength extension (690 to 1400 nm 2-P excitation range).
  • 2x PMT Non-descanned detectors, BiG detector (Binary GAsP low light detectors 2 x sensitivity).
  • Filters include: DAPI/CFP/GFP/RFP and Second Harmonic Generation (SHG) detection.
    Multiphoton image, macrophages (macgreen) in cardiac muscle (Blue, SHG)
    Multiphoton image, macrophages (macgreen) in cardiac muscle (Blue, SHG)
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