Multiphoton (2-photon) Microscopy

The Multiphoton Intravital Microscope is located within Lab G.069.

Multiphoton Microscopy sees deeper into live tissue (incubation for in vitro slices also available) and is less phototoxic than pinhole-based confocal microscopy. To make use of this technology, contact Bob Fleming in the first instance.

Zeiss LSM7MP upright Multiphoton Microscope.
Coherent Chamaeleon Ultra II Ti:Sa tunable laser with OPO wavelength extension (690 to 1400 nm 2-P excitation range).
2x PMT Non-descanned detectors, BiG detector (Binary GAsP low light detectors 2 x sensitivity).
Filters include: DAPI/CFP/GFP/RFP and Second Harmonic Generation (SHG) detection.

Multiphoton image, macrophages (macgreen) in cardiac muscle (Blue, SHG)

This article was published on 31 Oct, 2017