Expertise

In addition to all standard molecular biology techniques, members of the RICE group have expertise in a number of procedures.

1. In ovo manipulation of chicken embryos

• bead implantation
• microdissection
• cell and tissue grafting
• cell and tissue transplants
• application of growth factors and pathway inhibitors
• siRNA knock-down
• Antagomir suppression
• in ovo electroporation
• lenti virus expression

2. Generation of transgenic birds

• Lentivirus injection
• PGC editing

3. Primordial Germ Cell (PGC) culture

4. Chicken embryo fibroblast (CEF) isolation and culture

5. Organ culture

• Skin
• Gonad

6. Embryo culture

• ‘New’ Technique
• EC (easy) Technique

https://link.springer.com/protocol/10.1007%2F978-1-60327-483-8_17#page

7. Microscopy

• Super resolution microscopy
• Electron microscopy
• Confocal microscopy
• Optical projection Tomography
• Time-lapse

8. Lenti-virus production

9. Immunohistochemistry

10. Whole mount RNA in situ hybridisation

11. Sequencing from ancient and fixed tissues

12. In vitro CRISPR gene editing

13. miRNA analysis