X-Clarity tissue clearing allows large, intact histological samples to be rendered transparent for fluorescent labelling and microscopy. A problem that faces many people during imaging is the scattering of light through opaque or semi-opaque thick tissues by lipid membranes: the deeper you go, the less you see. A simple solution is to turn your sample transparent. The X-Clarity system clears your biological sample in three steps: Hydrogel Infusion following fixation (24h) Tissue to hydrogel hybridisation (3h) Lipid removal in a designated cassette Specifications The Facility offers the following cassettes for lipid removal: Very Large sample (two rat brain or several adult zebrafish). Well size: 35x53x20mm Large samples the size of a mouse brain. Well size: 16.8x16.8x20m 48 small samples, the size of a E11 mouse embryo. Well size: 6.5x6.5x10mm 192 small samples, the size of a 4 days old zebrafish embryo. Well size: 3x3x10mm You are then free to stain your tissue appropriately. Duration of lipid removal is sample dependent: the larger and more lipid rich, the longer it takes. Clearing is paid by cassette and by clearing time (in hours). 1 hour clearing costs £26 with £2 increment per extra hour clearing time. X-clarity website Booking Send an e-mail to the Facility describing your samples, cost codes. Once your job is accepted, bring your sample to the Facility and we will clear them for you. e-mail for a booking Mounting Medium For optimal transparency, we recommend that you use the X-Clarity mounting medium which we supply at £120 per 25ml vial This article was published on 2024-02-26
