Location, cost, access and specifications of PRIMO. Introduction PRIMO is a micropatterning system mounted on a Nikon Ti2. It allows for the exquisite patterning and sculpting of cell substrate with a precision down to 2 micrometers. Further information is available from https://www.alveolelab.com/our-products/primo-micropatterning/ Training: For training on PRIMO, contact us at CRM-Imaging@ed.ac.uk Cost: Price for usage is £24/h Location: Centre for Regenerative Medicine, ground floor In the UK, PLPP reagent (liquid or gel) for PRIMO can be bought by contacting Cairn Research (sales@cairn-research.co.uk). Book PRIMO via PPMS Micropatterning: manipulating substrate composition Micropatterning relies on the specific degradation of an anti-fouling reagent to uncover specific area of the substrate. These areas, either plain or gradients, can then be coated with proteins. Seeded cells preferentially bind the protein-coated substrate. The composition of the substrate is therefore specifically manipulated. Image Image courtesy of Alveole Substrate sculpting: manipulating substrate shape PRIMO allows to polymerize, degrade or cut gels down to 2um precision. The shape of the substrate can therefore be designed. You can construct niches for your stem cells, labyrinth for chemotactic experiments, micro wells for seeding organoids… Image Image courtesy of Alveole Reproducible microfabrication of substrate: shape and composition By combining micropatterning and substrate sculpting, PRIMO provides complete control over your cell culture substrate. Image Image courtesy of Alveole This article was published on 2024-02-26
