The facility is equipped with 3 state-of-the-art multi-laser cell analysers. Flow cytometry is a specialised laser-based single cell technology that measures certain physical and chemical characteristics of single particles or cells in suspension. This allows us to identify, analyse and separate (sort) certain cell populations of interest for downstream applications such as cell expansion, functional analyses or further DNA/RNA characterisation. The facility runs 3 state of the art multi-laser cell analysers: BD LSRFortessa (4 laser) analyser, BD LSRFortessa (5 laser) analyser and Acea Novocyte 3000 analyser. In addition, the facility also has 2 analysis workstations (located in G50) available for use – one with BD FACS Diva software, and the other with FlowJo and FCS Express software; these are used for post-acquisition analysis of data. All instruments and workstations can be booked through the PPMS booking system (https://ppms.eu/ed; registered users only, please contact the facility for access and more information). PPMS booking system For more information on services, charges, access to the facility (including analyser training) and for advice in protocol design, please contact facility staff. BD LSR Fortessa (4 laser) analyser A cell analyser composed of four lasers. BD LSR Fortessa (5 laser) analyser A cell analyser composed of 5 lasers. This article was published on 2024-02-26
