A glossary of terms related to stem cell research and regenerative medicine.
Adult stem cell
See tissue stem cell.
Describes the relationship of a tissue (or cell) donor and recipient as two genetically different individuals. Most tissue donations are allogeneic unless the donor and recipient are identical twins (see syngeneic).
The process of cell death that is controlled by a cell death biological pathway. It is also commonly called 'programmed cell death'.
Describes tissues (or cells) that were originally extracted or derived from a patient's body and can be used for treatment of disease. Often autologous cell treatments require ex vivo growth to increase the number of cells available before they are used.
An early stage embryo that is formed approximately 5 days after fertilisation of a human egg and is made of roughly 150 cells.
Cancer stem cells
Cells found in some forms of cancerous tumours that are self-renewing and multipotent. It is thought that these cells are responsible for tumour growth.
The smallest unit of life and the building block for most larger (multi-cellular) organisms. A cell contains genetic information in a nucleus and a membrane to separate the internal components of the cell from the outer environment.
Storage facilities for collections of carefully defined cells and cell lines. Many different types of cells, from human stem cells to insect cells, are cryogenically preserved, stored in cell banks and later thawed for use in medicine or research.
The process of growing cells in an in vitro (laboratory) setting.
The process of a cell dividing itself to create two new cells. A 'parent cell' must duplicate all genetic material and cell components before dividing into two 'daughter cells'.
A population of cells grown in vitro that have a specific genetic background (genotype) and physical characteristics (phenotype).
The movement of cells, often in response to an environmental stimulus (physical or chemical).
The natural process of tissue stem cells replacing cells within the body, often because they have become worn, damaged or have died.
The changing of a specialized cells function. Cell reprogramming can occur naturally in some organisms. The term is most often used in reference to laboratory manipulation of specialized cells, such as skin cells, to create iPS cells or other progenitor cell types.
Medical treatments that use cells as the active agent (opposed to drugs) to assist in healing a patient. Cell therapies are a form of regenerative medicine.
Central nervous system (CNS)
The brain and spinal column compose the central nervous system.
Medical treatments that involve the use of chemical compounds. Though chemotherapies can be used for many reasons, they are most often associated with cancer treatments.
The process of turning scientific knowledge and discoveries into clinical treatments. This involves everything from scientific research, pre-clinical trials, clinical trials and product development.
A series of tightly regulated studies to test the safety and efficacy of new therapies. All new medical treatments must go through clinical trials before being given governmental approval for widespread use.
A genetically identical copy of a cell. The term 'clone' is also used to refer to a whole organism derived from a cell of another organism.
The process of creating a clone, a genetically identical copy of a cell.
See experimental control.
Cord blood stem cells
Stem cells that are isolated from the blood of the umbilical cord.
The clear outer surface of the eye, which protects the pupil, iris, lens and other inner parts of the eye from the external environment. It is created by limbal stem cells.
The process of preserving tissues and cells for later use by cooling and storing them at very low temperatures, often in liquid nitrogen at -135°C or colder. Also known as 'cryopreservation.'
The liquid containing nutrients and other factors used to facilitate growth and maintenance of cells in the laboratory.
The loss of specialized characteristics of a cell.
The biological process of a cell dividing, differentiating and growing to creating complex tissues, organs, and ultimately a whole organism. The developmental process is what allows a fertilized egg to grow and mature into an adult.
The process of gaining specialised cellular functions by an immature cell. Fully differentiated cells (or terminally differentiated cells) cannot further alter their specialised functions.
Molecules found in all living cells that contain all the information for life. Information in DNA is coded within its chemical structure. Formally DNA is called deoxyribonucleic acid.
A study in which both the clinicians and patients involved do not know if they are giving/receiving an experimental control treatment or the treatment being investigated. These are the 'gold standard' for clinical trial tests because they prevent biased assessment.
The outermost layer of the three types of differentiated cells found in a two-week-old embryo. Ectodermal cells develop into tissues including the skin and nervous system.
The stages of development during the first eight weeks of pregnancy; from the first cell division of the fertilised egg until becoming a foetus.
Embryonic stem cells
The pluripotent cells derived from the inner cell mass of the blastocyst.
The innermost layer of the three types of differentiated cells found in a two-week-old embryo. Endodermal cells develop into organs for breathing and digesting, including the lungs, stomach, liver and intestines.
Epiblast stem cells
A type of stem cell derived from early post-implantation embryo that shares some properties with embryonic stem cells, such as the ability to produce all the differentiated cell types of the body. EpiSCs are similar to pluripotent embryo cells that are just about to choose a differentiation pathway.
Epidermal stem cells
Tissue stem cells found in skin (epidermis) that generate the many different cells that form skin.
The growth of cells in the laboratory that have been extracted from a person or organism. Growth conditions are typically chosen to minimize cell alterations.
A standard or benchmark measurement which is used to evaluate the outcome of an experiment. The most common form of experimental control is a 'negative control', where the item to be tested is omitted and the expected result is no change at all.
The developmental period after the embryo stage of development until birth. The foetus resembles a newborn baby and has formed most major tissues and organs.
Manipulation of the genetic components of cells for the purpose of medical treatment. Often the goal of gene therapy is to fix natural mutations that lead to disease.
Segments of DNA that are associated with hereditary traits passed from parent to offspring. Genes encode information to make RNA and proteins, which allow cells to perform specialized functions.
Active production by a cell of the gene product. The product could be either an RNA molecule or a protein that is encoded by the gene. The production of a gene product is often regulated by other proteins. Genes are often regulated by transcription factors.
Anything relating to how genes and DNA allow hereditary traits and information to be passed on from one generation to another.
Genetically modified organism (GMO)
Any living organism that contain artificially altered genetic material.
The genetic material of an organism. It is encoded either in DNA or, for many types of viruses, in RNA.
The specific DNA sequence (genetic background) of a living organism. The term 'genotype' describes the entire genetic background of an organism, but often researchers use the term to refer to a specific inherited mutation in a gene.
A natural protein that stimulates cells to multiply.
The formation of different cells that make up blood.
Haematopoietic stem cells
Tissue stem cells responsible for generating cells found in our blood and immune system.
The specialised cell type of the liver. These cells are primarily responsible for making bile for digestion, regulating sugar levels in the blood, detoxifying metabolic waste, and making proteins for blood plasma.
Immortal cell line
A cell line that has the ability to undergo cell division indefinitely, also known as a 'continuous cell line'. Immortal cell lines permit researchers to work with the same cells in vitro for long periods of time and to greatly amplify the number of cells in culture.
Biological processes, organs, tissues and cells that provide the body with resistance to infections. The immune system primarily works by destroying anything that is sees as foreign to the body.
The failure of a patient's body to acquire and integrate transplanted tissue, often due to a detrimental reaction by the immune system.
A biological process or experiment that takes place under controlled laboratory conditions, outside of a living organism.
A biological process or experiment that occurs within a living organism.
Induced pluripotent stem (iPS) cells
Stem cells created from fully differentiated tissue cells that have been artificially stimulated to become pluripotent cells similar to embryonic stem cells.
Inner cell mass
A cluster of pluripotent cells found within a blastocyst from which embryonic stem cells can be derived.
Limbal stem cells
Tissue stem cells responsible for generating the cornea of the eye.
See clinical translation.
Mesenchymal stem cell
A multipotent stem cell derived from bone marrow or fat that has the ability to differentiate into many (but not all) cell types that make bone, tendon, fat, cartilage, and others tissues.
The middle layer of the three types of differentiated cells found in a two-week-old embryo. Endodermal cells develop into internal organs, such as skeletal muscle, bone, cartilage, blood, veins and arteries.
Stem cells with the ability to generate a subset of specialised cell types, typically found within the same organ.
A genetic disorder causing muscles to become weak and waste away, greatly hindering movement.
A group of disorders that cause a lack of muscle control due to the loss of signals from unhealthy or dying nerve cells.
A specialised cell that can rapidly send electrical signals. Neurons are found in the brain, spinal cord and throughout the rest of the body. They allow for sensory reception, thought processes, consciousness, movement and other functions.
A group of specialized tissues organised to form a distinct structure in the body and work together to carry out functions needed for the whole organism. Examples include the lungs, heart, liver, etc.
Any disease-causing factor or microorganism.
Peripheral nervous system (PNS)
All neurons that are found outside of the brain and spinal cord.
Medical treatments that are custom tailored to treat the complication and meet the immunological needs of a patient.
The physical (observable) characteristics of a cell, tissue, or organism. These characteristics are the materialization of the information encoded by the genotype.
Stem cells with the ability to generate all the specialised cell types of an organism. Pluripotent cells can form the cells of an embryo, but lack the ability to create the supporting cells and tissues needed to permit the development of a whole organism.
During cell differentiation, this is the last distinguishable cell type before a cell is fully differentiated and specialised.
Primary cell culture
An in vitro culture of cells that was generated directly from in vivo tissue.
Principle Investigator (PI)
The researcher, clinician or scientist that takes overall responsibility for research work in a laboratory.
During cell differentiation from a stem cell to a fully differentiated specialised cell, several distinguishable cell types may arise. Progenitor cells are these in-between cells that are more specialised and come after stem cells, but precede the final specialised cell state.
The multiplying of cells through repeated cell division.
Medical treatments that restore complete function to tissues and organs that are damaged by age, injury or disease. These treatments range from drugs that stimulate tissue regeneration to methods of cell and tissue replacement.
See cell reprogramming.
The ability of a cell to repeatedly undergo cell division and create new cells exactly like the original 'parent' cell. This is one of the defining properties of stem cells.
See specialised cells.
Somatic stem cells
See tissue stem cells.
Cells that are fully differentiated and have specific roles within a tissue or organ. Examples include the ability of muscle cells to contract and stomach cells to produce acid for digestion.
Cells that have the ability to both self-renew and differentiate. Stem cells are critical for development, growth, cell renewal and healing.
Stem cell line
Cells that have been well characterized, have specific phenotypes, and can be indefinitely grown in the laboratory. These cells often are used for experimentation because they are homogenous and are used in other laboratories. This permits researchers to easily conduct experiments on the same cells and more accurately compare experimental results.
When a tissue (or cell) donor and recipient are genetically identical. This is only possible in the situation that the donor and recipient are identical twins.
A tumor composed of a chaotic mixture of many tissue or organ components, that is caused by the abnormal presence of pluripotent cells, which multiply and differentiate without the normal controls imposed by the embryo.
Terminally differentiated cells
Cells that cannot undergo changes in their identity or specialised functions.
A group of cells that perform similar specialised functions.
Tissue stem cells
Tissue stem cells can both self renew and give rise to at least one more specialised (differentiated) cell type. They can be unipotent, meaning that they only make one type of specialised cell (eg the spermatogonial stem cell) or multipotent, meaning they can generate several types of specialised cell. Under normal circumstances, tissue stem cells generate only the cell types that make up the or organ / tissue system they reside in. Also known as 'adult' or 'somatic' stem cells.
Cells with the ability to undergo development into complete organisms. An example of such a cell is the zygote.
Proteins that bind to DNA, often at or near the beginning of a gene, and regulate the amount of RNA or protein produced that is coded by a gene. Transcription factors can both increase or decrease the gene expression (the amount of gene products a cell produces).
An organism that contains artificially introduced genetic material.
Abnormal tissue growth that is caused by unregulated and rapidly multiplying cells.
A cell that only has the ability to differentiate into a single specialised cell type. This is often the case of precursor cells.
A totipotent cell formed by the unification of a sperm and egg.