Fernando De La Cuesta
Marie Curie Research Fellow
Past PhD students supervised
Ms Ilaria Passalacqua
Research summary
I lead a project funded by the EU Comission studying the role of extracellular vesicles in pulmonary hypertension. I am a collaborator in projects of proteomics applied to gene therapy viral vectors, Single-Cell RNA-Seq and lncRNAs.
Current research interests
The main aim of my research is to study extracellular vesicle mediated cell-to-cell communication between human smooth muscle cells and endothelial cells, evaluate its relevance in vascular injury in pulmonary arterial hypertension, and determine the significance of long non-coding RNA in this crosstalk. For imaging EV transfer among vascular cells, I have optimised a pioneer approach based on Cre-loxP recombination which results in a fluorescent colour switch of cells upon EV uptake. The RNA cargoes present in EVs are being analysed by RNA-Seq. With the combination of both imaging and molecular approaches we are unvealing the biological relevance of EVs in pulmonary arterial hypertension.Current project grants
EU Comission Marie Skłodowska-Curie Actions Individual Fellowship 2016 (EV-LNC)
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Transcriptional dynamics of pluripotent stem cell-derived endothelial cell differentiation revealed by single-cell RNA sequencing
In:
European Heart Journal, vol. 41, pp. 1024–1036
DOI: https://doi.org/10.1093/eurheartj/ehz351
Research output: Contribution to Journal › Article (Published) -
Extracellular vesicle cross-talk between pulmonary artery smooth muscle cells and endothelium during excessive TGF-β signalling: implications for PAH vascular remodelling
In:
Cell communication and signaling, vol. 17
DOI: https://doi.org/10.1186/s12964-019-0449-9
Research output: Contribution to Journal › Article (Published) -
Human adenovirus serotype 5 is sensitive to IgM-independent neutralization in vitro and in vivo
In:
Viruses, vol. 11
DOI: https://doi.org/10.3390/v11070616
Research output: Contribution to Journal › Article (Published) -
Citric Acid Metabolism in Resistant Hypertension: Underlying Mechanisms and Metabolic Prediction of Treatment Response
In:
Hypertension
DOI: https://doi.org/10.1161/HYPERTENSIONAHA.117.09819
Research output: Contribution to Journal › Article (Published) -
Immune system deregulation in hypertensive patients chronically RAS suppressed developing albuminuria
In:
Scientific Reports, vol. 7
DOI: https://doi.org/10.1038/s41598-017-09042-2
Research output: Contribution to Journal › Article (E-pub ahead of print) -
Kalirin and CHD7: Novel endothelial dysfunction indicators in circulating extracellular vesicles from hypertensive patients with albuminuria
(10 pages)
In:
Oncotarget, vol. 8, pp. 15553-15562
DOI: https://doi.org/10.18632/oncotarget.14948
Research output: Contribution to Journal › Article (Published) -
iTRAQ proteomic analysis of extracellular matrix remodeling in aortic valve disease
In:
Scientific Reports, vol. 5, pp. 17290
DOI: https://doi.org/10.1038/srep17290
Research output: Contribution to Journal › Article (Published) -
Kidney tissue proteomics reveals regucalcin downregulation in response to diabetic nephropathy with reflection in urinary exosomes
In:
Translational Research, vol. 166, pp. 474-484.e4
DOI: https://doi.org/10.1016/j.trsl.2015.05.007
Research output: Contribution to Journal › Article (Published) -
Prediction of development and maintenance of high albuminuria during chronic renin-angiotensin suppression by plasma proteomics
(8 pages)
In:
International Journal of Cardiology, vol. 196, pp. 170-7
DOI: https://doi.org/10.1016/j.ijcard.2015.05.148
Research output: Contribution to Journal › Article (Published) -
KLK1 and ZG16B proteins and arginine-proline metabolism identified as novel targets to monitor atherosclerosis, acute coronary syndrome and recovery
(12 pages)
In:
Metabolomics : Official journal of the Metabolomic Society, vol. 11, pp. 1056-1067
DOI: https://doi.org/10.1007/s11306-014-0761-8
Research output: Contribution to Journal › Article (Published) -
Lipid and protein maps defining arterial layers in atherosclerotic aorta
(4 pages)
In:
Data in brief, vol. 4, pp. 328-31
DOI: https://doi.org/10.1016/j.dib.2015.06.005
Research output: Contribution to Journal › Article (Published) -
Molecular anatomy of ascending aorta in atherosclerosis by MS Imaging: Specific lipid and protein patterns reflect pathology
(7 pages)
In:
Journal of proteomics, vol. 126, pp. 245-51
DOI: https://doi.org/10.1016/j.jprot.2015.06.005
Research output: Contribution to Journal › Article (Published) -
Identification of a circulating microvesicle protein network involved in ST-elevation myocardial infarction
(11 pages)
In:
Thrombosis and Haemostasis, vol. 112, pp. 716-26
DOI: https://doi.org/10.1160/TH14-04-0337
Research output: Contribution to Journal › Article (Published) -
Proteomic characterization of human coronary thrombus in patients with ST-segment elevation acute myocardial infarction
In:
Journal of proteomics, vol. 109, pp. 368-381
DOI: https://doi.org/10.1016/j.jprot.2014.07.016
Research output: Contribution to Journal › Article (Published) -
Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis
(11 pages)
In:
Journal of proteomics, vol. 96, pp. 92-102
DOI: https://doi.org/10.1016/j.jprot.2013.10.037
Research output: Contribution to Journal › Article (Published) -
Identification of a urine metabolomic signature in patients with advanced-stage chronic kidney disease
(9 pages)
In:
Kidney International, vol. 85, pp. 103-11
DOI: https://doi.org/10.1038/ki.2013.328
Research output: Contribution to Journal › Article (Published) -
Modification of the secretion pattern of proteases, inflammatory mediators, and extracellular matrix proteins by human aortic valve is key in severe aortic stenosis
(14 pages)
In:
Molecular & Cellular Proteomics (MCP), vol. 12, pp. 2426-2439
DOI: https://doi.org/10.1074/mcp.M113.027425
Research output: Contribution to Journal › Article (Published) -
Deregulation of smooth muscle cell cytoskeleton within the human atherosclerotic coronary media layer
(11 pages)
In:
Journal of proteomics, vol. 82, pp. 155-65
DOI: https://doi.org/10.1016/j.jprot.2013.01.032
Research output: Contribution to Journal › Article (Published) -
Secretome of human aortic valves
(7 pages)
DOI: https://doi.org/10.1007/978-1-62703-386-2_19
Research output: › Chapter (Published) -
Characterization and analysis of human arterial tissue secretome by 2-DE and nLC-MS/MS
(10 pages)
DOI: https://doi.org/10.1007/978-1-62703-405-0_7
Research output: › Chapter (Published)