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Gene study charts mutation effects

Scientists have mapped how thousands of genetic mutations can affect a cell’s chances of survival.

The study involving yeast reveals how different combinations of mutations in a single gene can influence whether the cell lives or dies.

Graph plotting survival impact of genetic mutations reveals red and green tartan pattern
This tartan-like pattern reveals how multiple genetic mutations within a single gene influence whether the cell lives or dies. Mutations that cancel each other out to preserve survival are highlighted in red. Those that work together to reduce chances of survival are green.

Mutations

It is the first time scientists have been able to measure the effects of every possible combination of mutations in a gene.

Researchers say the technique used in their research could aid studies into the effects of gene mutations that are linked to diseases in people.

Yeast study

The team from the University of Edinburgh produced 60,000 strains of yeast, each with a different combination of mutations in a single gene.

They then watched the cells to see what effect the mutations had on survival and whether different combinations of genetic changes helped the yeast to fare better or worse.

Survival

In some cases, the effects of different mutations cancelled each other out and the cells survived. The effects of other mutations added together to greatly reduce the cells’ chances of survival.

Genetic changes that have the greatest combined effect on survival tend to be located close to each other in the three-dimensional structure of the genetic material, the study found.

Shape 

This means that the technique could help scientists to predict the shapes of molecules encoded in our genes.

The research, published in the journal Science, received funding from the Medical Research Council (MRC) and the Wellcome Trust.

We pitted 60,000 mutated yeast strains against each other in a fight for survival. Those that survived were able to produce more copies of themselves and dominate the population. This is evolution in action.

Dr Grzegorz KudlaMRC Human Genetics Unit

 

Cells without any mutations fared the best and reproduced faster than any of the mutated strains, which tells us that this particular gene has been optimally configured by evolution.

Dr Olga PuchtaMRC Human Genetics Unit