MRC Human Genetics Unit
Medical Research Council Human Genetics Unit

APPLY NOW - 3 year PhD project

Study of pioneer transcription factor dynamics during vertebrate Zygotic Genome Activation

Dimitrios Papadopoulos Research Project Image

Project Description

How do transcription factors (TFs) regulate development at the correct time, dosage and place? How do they “know” how to “find” their target sites in live cells, with ultimate precision and specificity? Many developmental disorders are caused by haploinsufficiencies of TFs, thus their concentration and molecular behaviour is critical for directing correct embryonic development. However, how do TF concentration, chromatin-binding kinetics and cell-to-cell heterogeneity (noise) regulate function and specification in live tissue temporally/spatially?

We will use advanced genetic tools and microscopy-based methodology to tackle these very fundamental questions at the single-molecule level. We will investigate how cell-to-cell variability in pioneer TF concentration and their chromatin binding kinetics in live zebrafish embryos determine the robustness and temporal precision of Zygotic Genome Activation. We will also study what developmental consequences the absence of such control has during early embryogenesis.

Dimitrios Papadopoulos Research Group

Application guidelines

Applicants should hold at least an upper second class degree in a relevant subject and comply with English language requirements (see application page).  

Application Deadline: 18th January 2019

  • Click the following link to apply for the project

Apply now