Institute of Genetics and Cancer

DNA Sequencing Facility

Sequencing facility, data and analysis.

DNA sequencing facility

An in house DNA sequencing facility providing Sanger and 2nd generation sequencing to all IGMM researchers and their collaborators, operated by two experienced sequencing technologists.

  • Sample numbers ranging from single tube through to 384 well plates can be submitted to the facility.  
  • Provides an automated PCR purification service directly before Sanger Sequencing.
  • Plasmid mini-prep extraction and isolation service processed by liquid handling robots.  
  • DNA and RNA quality and quantitation is available via Tapestation/Bioanalyzer.
  • Human Cell Line Authentication using STR profiling.

Instrumentation

 
Sanger Instruments Capillaries
3130xl 16
3730xl 96
2nd Generation Instruments Data (Gb)
Ion Proton 10-200
Liquid handling instruments Pipette channels
BioMek NX (x2) 1, 8 and 96
DNA/RNA Qualititative and Quantitative Analysers Chip Type and Sensitivity range
Agilent 2100 DNA 1000; DNA High Sensitivity
  RNA Nano; RNA Pico
Agilent 4200 TapeStation DNA 1000; DNA 5000; RNA

People

 
Stewart McKay Facility Manager
Stephen Brown Sequencing Technologist
Jeffrey Joseph Sequencing Technologist

Contact

For more information on the services provided within the facility, please contact:

sequence@igc.ed.ac.uk

 

DNA sequencing image