Centre hosted facilities
Details of facilities located within the MRC Centre for Inflammation Research
QMRI Flow Cytometry and Cell Sorting Facility
The Queen’s Medical Research Institute Flow Cytometry and Cell Sorting Facility provides a comprehensive service to all researchers requiring the use of flow cytometry technology and its many associated techniques. The facility allows researchers access to advanced cell sorting instrumentation and flow cytometry analysers, and is fully supported by highly trained facility staff.
We are available to provide understanding of the principles behind the technology, training in the use of the equipment and relevant software and, troubleshooting of the instruments. As well as all aspects of technical assistance, we are able to advise researchers on experimental design, sample preparation and data analysis.
Please contact us if you are interested in using the facility services to benefit your research or if you have any questions relating to this technology.
Shonna Johnston - Manager (email)
Will Ramsay - Cytometry Specialist (email)
Mari Pattison - Assistant
Confocal and Advanced Light Microscopy Facility (CALM)
The Queen’s Medical Research Institute imaging facility Confocal and Advanced Light Microscopy is a multi-user facility, hosted in the MRC Centre for Inflammation Research . The CALM facility is open to all University researchers and offers access to high-end light microscopy platforms for fluorescence based multi-dimensional imaging. The facility has two areas: CALM_core located in the CIR, which houses two confocal microscopes and a suite of light microscopes for bright-field and fluorescence imaging; and CALM_live, in the QMRI basement, which houses a spinning disk confocal and a multi-photon imaging platform, both of which are designed for live specimen/animal imaging. CALM works in collaboration with the Flow Cytometry Facility and access to the facility/training on the systems can be arranged through contacting:
Trudi Gillespie - Confocal and Advanced Light Microscopy Facility Manager (email)
Rolly Wiegand - Confocal and Advanced Light Microscopy Facility Head/Academic lead (email)
Shonna Johnston - Flow Cytometry Facility Manager (email)
The Edinburgh Phenotypic Assay Centre
A joint initiative of the School of Biological Sciences and the College of Medicine and Veterinary Medicine, is located in E2.25 and E2.26 of the Queen's Medical Research Institute.
Under the direction of Professors Neil Carragher, Margaret Frame and David Gray, the EPAC works to link clinical and basic research groups with the latest innovations in automated imaging, image-informatics, biological modelling and assay screening standards. EPAC scientists develop state-of-the-art cell-based assays suitable for medium- to high-throughput screening utilising chemical and natural product as well as si/sh/sg RNA and CRISPR libraries.
In addition to developing their own high throughput-ready screens for specific in-house research, EPAC scientists collaborate with both academic researchers and pharmaceutical partners. They provide guidance and training to help academic researchers translate their novel experiments into high throughput screening assays in which the pharmaceutical industry may be interested and collaborate with researchers to perform screens in an academic setting.
The EPAC, alongside the Target Discovery Institute of University of Oxford and the National Phenotypic Screening Centre based at University of Dundee, serves as the third major academic hub within the Phenomics Discovery Initiative , a public private consortium that aims to enhance translation of phenotypic biology into novel therapeutics for patients.