Dr Nila Roy Choudhury
I currently work on the E3 ubiquitin ligase Trim25, a novel RNA binding protein, that has an important role in innate immunity.
I did my undergraduate degree in Biomedical Laboratory Science at the Karolinska Institute in Sweden. Following this I acquired a PhD in Molecular Virology at the University of Edinburgh. Since then I have been working as a postdoc in the Michlewski group.
I currently work on the E3 ubiquitin ligase Trim25, a novel RNA binding protein, that has an important role in innate immunity. I am exploring the functional importance of its RNA binding ability in cell biology and during viral infection.
Kooshapur, H., Choudhury, N.R., Simon, B., Mühlbauer, M., Jussupow, A., Fernandez, N., Jones, A.N., Dallmann, A., Gabel, F., Camilloni, C., Michlewski, G*., Caceres, J.F*., and Sattler, M*. Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1. (2018) Nat Commun. 2018 9:2479 (* - co-corresponding)
Choudhury, N.R., and Michlewski, G. Quantitative identification of proteins that influence miRNA biogenesis by RNA pull-down-SILAC mass spectrometry (RP-SMS). (2018) Methods 18:30052-5.
Choudhury, NR., Heikel, G., Trubitsyna, M., Kubik, P., Nowak, JS., Webb, S., Granneman, S., Spanos, C., Rappsilber, J., Castello, A., and Michlewski,G. RNA-binding activity of Trim25 is mediated by its PRY/SPRY domain and is required for ubiquitination. (2017) BMC Biology 15:105
Nowak, J.S., Hobor, F., Downie Ruiz Velasco, A., Choudhury, N.R., Heikel, G., Kerr, A., Ramos, A., and Michlewski, G. Lin28a uses distinct mechanisms of binding to RNA and affects positively and negatively miRNA levels. (2017) RNA 3:317-332
Choudhury, N.R., Nowak, J.S., Zuo J., Rappsilber, J., Spoel H.S., and Michlewski, G. Trim25 is an RNA-specific activator of Lin28a/TuT4-mediated uridylation. (2014) Cell Rep. 4:1265-72.
Nowak, J.S., Choudhury, N.R., de Lima Alves, F., Rappsilber, J., and Michlewski, G. Lin28a regulates neuronal differentiation and inhibits miR-9 processing. (2014) Nat Commun. 5:3687
Choudhury, N.R., de Lima Alves, F., de Andrés-Aguayo, L., Graf, T., Cáceres, J.F., Rappsilber, J., and Michlewski, G. Tissue-specific control of brain-enriched miR-7 biogenesis. (2013) Genes Dev. 27: 24-38. Evaluated by Faculty of 1000 Biology