Infection Medicine

PhD project available in Templeton Lab

Development of a rapid, genome sequence-based, diagnostic approach for rapid diagnosis to optimise management of infections

Abstract

Rapid identification of the etiologic agent of an infectious disease is essential for best management, setting up treatment and preventive measures. The current approach requires that specific pathogen identification is performed by direct diagnostic tests which normally include culture and PCR-based assays. Although these approaches are highly specific and well validated, they suffer a number of limitations. In addition many of the current techniques are relatively slow taking several days to deliver results  and often are biased to one or two pathogens and don’t capture the complexity of the full microbial picture.

There are now new novel technologies represented by next generation sequencing (NGS) that can address current diagnostics limitation. The MinION (Oxford Nanopore Technologies, Oxford, UK), a novel portable real-time NGS sequencer, enables the application of sequencing for rapid diagnosis with inexpensive sample preparation even in low-throughput laboratories. While it has potential this is not yet the case and is a unique opportunity to fulfil a diagnostic gap for the future.

The aim of this PhD project is to develop a rapid, genome sequence-based, diagnostic approach for rapid diagnosis to optimise management of infections. The project focuses on:

  • Challenging cases
  • Rapid delivery at point of impact
  • Poly microbial diagnosis

Please contact the project supervisor Dr Kate Templeton if you have any questions about the project.

Further Information

Dr Kate Templeton