Easter Bush Science Outreach Centre

PCR Masterclass: A Question of Taste

In collaboration with the Centre for Open learning, we are running our full day, hands-on polymerase chain reaction (PCR) masterclass as part of their short course programme.

Learning Level: Adult (16+ years old)

LocationCharnock Bradley Building Reception, 10 minutes before the workshop start time

Availability:  Thursday 4th June 2020, 10:00am - 3:00pm (organised by Centre for Open Learning)

Minimum attendance: 16   

Maximum attendance: 24

Cost: £15 

Duration: 5 hrs

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Workshop Description

In this laboratory-based workshop, you will determine whether your version of the “bitter taste” receptor gene (your genotype) matches your ability to taste a bitter compound (your phenotype). Using cutting-edge equipment, you’ll carry out the modern laboratory techniques of DNA extraction, PCR, restriction enzyme digestion and DNA gel electrophoresis.

This course is only available to those who have completed “Introduction to Cell Biology” or “Higher Biology” / an equivalent.

Course Details

Pre-requisites for enrolment

Students must have studied either the Short Course, “Introduction to Cell Biology” or Biology at SCQF level 6 (such as Higher Biology)

Content of Course

This workshop will allow you the chance to use the state-of-the-art laboratory at the Easter Bush Science Outreach Centre (EBSOC).

The EBSOC team will guide you through activities designed to help you gain an understanding of the PCR technique.

Laboratory skills include:

  • DNA extraction
  • Centrifugation
  • Micropipetting
  • Polymerase Chain Reaction (PCR)
  • Restriction digest
  • DNA gel electrophoresis

The session will close with a discussion involving researchers from The Roslin Institute.

In order to attend this workshop, students should have an existing knowledge of cell biology, from a course such as “Introduction to Cell Biology” or Higher / A-level Biology.

Teaching method(s)

Laboratory-based workshop

Learning outcomes

On completion of this course participants will be able to:

  1. Use restriction enzymes and electrophoresis to detect single nucleotide polymorphisms (SNPs) in their own DNA
  2. Interpret their experimental results
  3. Safely use laboratory equipment

Queries

If you have questions regarding the course or enrolment, please contact COL Reception at Paterson's Land by email COL@ed.ac.uk or by phone 0131 650 4400.

Accessibility and Inclusion:

We are keen to make our events as inclusive as possible - please email us at ebsoc@ed.ac.uk or call the Easter Bush Science Outreach Centre team on 0131 651 9679 before booking if you would like to find out more about accessibility or usability.

Risk Assessment