Easter Bush Science Outreach Centre

DNA & the Genome| PCR Masterclass: A Question of Taste

A full day, hands-on polymerase chain reaction (PCR) masterclass. Using their own DNA, students will discover and compare their version of a bitter taste receptor gene (genotype) with their ability to taste the bitter compound (phenotype). Includes a unique opportunity to meet and work with our scientists.  

Learning Level: Higher Biology & Human Biology

Location: Easter Bush Science Outreach Centre, The University of Edinburgh Easter Bush Campus, EH25 9RG

Minimum attendance: 16*

Maximum attendance: 24

Duration: 5 hours

Timings: 10am - 3pm**

Cost: £8 per student

 

Workshop Description

A full day, hands-on polymerase chain reaction (PCR) masterclass for Higher Biology and Higher Human Biology students.  Using their own DNA, pupils will discover and compare their version of a bitter taste receptor gene (their genotype) to their ability to taste the bitter compound (their phenotype). They will use cutting-edge equipment to carry out the modern laboratory techniques of DNA extraction, PCR, restriction enzyme digestion and DNA gel electrophoresis.

Learning Objectives

  • Understand DNA structure and function
  • Understand that we can identify genotypes using molecular biology techniques
  • Understand how restriction enzymes cut DNA
  • Use of restriction enzymes and electrophoresis to detect single nucleotide polymorphisms (SNPs) in their own DNA
  • Interpretation of experimental results
  • To reveal the world of work in scientific research

Techniques used

  • DNA extraction
  • Centrifugation
  • Micropipetting
  • Polymerase Chain Reaction (PCR)

  • Restriction digest
  • DNA gel electrophoresis

Workshop timeline

  • DNA extraction of student's own cheek cells using centrifugation to pellet their cells
  • Introduction to PCR and using micropipetting to set up PCR
  • Restriction digest to determine genotype
  • Electrophoresis on agarose gels
  • Analysis and interpretation of results
  • Discussion with scientists from The Roslin Institute 

Cirriculum Links

Higher Biology (2018-2019)
Higher Human Biology  (2018-2019)
1 The structure of DNA (a) Structure of DNA

2 Structure and replication of DNA

(a) Structure of DNA  (c) Polymerase chain reaction (PCR)
2 Replication of DNA  (b) Polymerase chain reaction (PCR)

3 Gene expression (e) Phenotype is determined by the proteins produced as the result of gene expression.

3 Gene expression (e) Phenotype is determined by the proteins produced as the result of gene expression.

4 Mutations (a) Mutations are changes in the DNA that can result an altered protein being synthesized (b) Single gene mutations

6 Mutations (a) Mutations (b) Single gene mutations

5 Human genomics (b) Individual genome analysis

8 Genomic Sequencing (c) Comparison of genomes from different species (d) Individual genome analysis

 

Availability

Bookings will open 1st June 2020

August September October
Wednesday 19th August 2020 Tuesday 8th September 2020 Thursday 1st October 2020
Thursday 20th August 2020 Wednesday 9th September 2020 Tuesday 6th October 2020
Tuesday 25th August 2020 Thursday 10th September 2020 Wednesday 7th October 2020
Wednesday 26th August 2020 Tuesday 15th September 2020 Thursday 8th October 2020
Thursday 27th August 2020 Wednesday 16th September 2020 Tuesday 13th October 2020
  Thursday 17th September 2020 Wednesday 14th October 2020
  Wednesday 23rd September 2020 Thursday 15th October 2020
  Thursday 24th September 2020 Wednesday 28th October 2020
  Tuesday 29th September 2020 Thursday 29th October 2020
  Wednesday 30th September 2020  

 

Important Notes

*   Please contact us ebsoc@ed.ac.uk if your group falls below the minimum as we may be able to accommodate your request through

      our small groups waiting list.

** A tour of The Roslin Institute can be organised on request, but this will affect the timings of your visit.

Downloadable Resources
Risk Assessment
Workshop Decription