Easter Bush Science Outreach Centre

DNA & the Genome| PCR Masterclass: A Question of Taste

A full day, hands-on polymerase chain reaction (PCR) masterclass. Using their own DNA, students will discover and compare their version of a bitter taste receptor gene (genotype) with their ability to taste the bitter compound (phenotype). Includes a unique opportunity to meet and work with our scientists.  

Learning Level: Higher, HNC, HND

Location: Easter Bush Science Outreach Centre, The University of Edinburgh Easter Bush Campus, EH25 9RG

Minimum attendance: 16*

Maximum attendance: 24

Duration: 5 hours

Timings: 10am - 3pm**

Cost: £8 per student

Please add me to the 2022/2023 waiting list!

Workshop Description

A full day, hands-on polymerase chain reaction (PCR) masterclass for Higher Biology and Higher Human Biology students.  Using their own DNA, pupils will discover and compare their version of a bitter taste receptor gene (their genotype) to their ability to taste the bitter compound (their phenotype). They will use cutting-edge equipment to carry out the modern laboratory techniques of DNA extraction, PCR, restriction enzyme digestion and DNA gel electrophoresis.

Learning Objectives

  • Understand DNA structure and function
  • Understand that we can identify genotypes using molecular biology techniques
  • Understand how restriction enzymes cut DNA
  • Use of restriction enzymes and electrophoresis to detect single nucleotide polymorphisms (SNPs) in their own DNA
  • Interpretation of experimental results
  • To reveal the world of work in scientific research

Techniques used

  • DNA extraction
  • Centrifugation
  • Micropipetting
  • Polymerase Chain Reaction (PCR)

  • Restriction digest
  • DNA gel electrophoresis

Workshop timeline

  • DNA extraction of student's own cheek cells using centrifugation to pellet their cells
  • Introduction to PCR and using micropipetting to set up PCR
  • Restriction digest to determine genotype
  • Electrophoresis on agarose gels
  • Analysis and interpretation of results
  • Discussion with scientists from The Roslin Institute 

Cirriculum Links

Higher Biology (2018-2019)
Higher Human Biology  (2018-2019)
1 The structure of DNA (a) Structure of DNA

2 Structure and replication of DNA

(a) Structure of DNA  (c) Polymerase chain reaction (PCR)
2 Replication of DNA  (b) Polymerase chain reaction (PCR)

3 Gene expression (e) Phenotype is determined by the proteins produced as the result of gene expression.

3 Gene expression (e) Phenotype is determined by the proteins produced as the result of gene expression.

4 Mutations (a) Mutations are changes in the DNA that can result an altered protein being synthesized (b) Single gene mutations

6 Mutations (a) Mutations (b) Single gene mutations

5 Human genomics (b) Individual genome analysis

8 Genomic Sequencing (c) Comparison of genomes from different species (d) Individual genome analysis

 

Availability

Please add me to the waiting list!

 

Important Notes

*   Please contact us ebsoc@ed.ac.uk if your group falls below the minimum as we may be able to accommodate your request through

      our small groups waiting list.

** A tour of The Roslin Institute can be organised on request, but this will affect the timings of your visit.

Downloadable Resources
Risk Assessment
Workshop Decription