New PNAS paper for Cousin lab reveals the “ADBE Proteome”
New PNAS paper for Cousin lab
This paper combined biochemical enrichment protocols with state of the art mass spectrometry to reveal the molecular inventory of activity-dependent bulk endocytosis (ABDE) for the first time.
ABDE is the dominant mode of synaptic vesicle endocytosis during intense neuronal activity, suggesting that it should play a vital role in brain events that rely on these patterns of communication. However this is still unknown, since no molecules have been identified that are specific to ADBE.
The discovery of the ADBE Proteome should allow scientists to decode how ADBE controls brain function via precise interventions that target ADBE-specific molecules.
The ADBE Proteome is the equivalent of the Rosetta Stone for understanding how this pathway controls brain function. It should provide a rich resource of new avenues to explore the role of this endocytosis mode in terms of its physiology and also potential pathophysiology in disorders of excess neuronal activity, such as epilepsy.
The study is published in the journal Proceedings of the National Academy of Sciences. It was funded by The Wellcome Trust.
Link to paper - http://www.pnas.org/content/early/2018/10/08/1809189115