Confocal and Advanced Light Microscopy (CALM) Facility

CALM Facility equipment finder

This page helps you to find the right CALM equipment for your experiments.

Due to present changes, this page is under development and will be updated in due course, thanks for your patience!

 

Finding the right microscope for your experiment

The choice of microscope depends on the type of sample and the following table gives you recommendations based on the most important imaging parameters. Please note that the imaging conditions might vary significantly depending on the sample and its quality. Thus, the given recommendations are for general guidance only, and different choices might be preferable. If you are not sure about the selection of the equipment, please contact the facility staff and we will provide in-depth technical advice.

 

CALM equipment table
Imaging parameter
Leica SP5
Leica SP8
Andor SDCM
Leica DMi8 basic
Zeiss Apotome
Zeiss LSM780
Zeiss P.A.L.M.
               
lateral resolution
+++
+++
+++
+++
+++
+++
+++
axial resolution
+++
+++
+++
-
-
+++
-
3D acquisition
+++
+++
+++
-
-
+
-
penetration depth
++
++
++
-
-
++
-
acquisition speed
+
+
+++
+
++
+
++
environmental control
+++
+
+++
-
++
-
-
gas supply (CO2/O2/N2)
5%/-/-
var/-/-
var/-/-
-/-/-
5%/-/-
-
-/-/-
excitation lines/filter bands
9
7
4
4
4
6
3
spectral channels/detection
7
7
4
4
4
6
3
automated stage
+
+
+
-
+
+
+
physical autofocus
-
-
-
-
+
-
-
spectral unmixing
+
+
-
-
-
+
-

var = variable (concentration or temperature)