TIRF
IMPACT hosts a Total Internal Reflection Fluorescence (TIRF) microscope coupled to an Olympus IX81 epi-fluorescence microscope.
TIRF system at IMPACT is integrated onto a standard epi-fluorescence system from Olumpus IX81 microscope. This is a fully motorised microscope controlled by Olympus CellR software and is equipped with a Hamamatsu EMCCD camera.
Key features:
- Suitable for TIRF imaging and/or standard epi-fluorescence imaging
- Fully motorised, automatic filter/objective switching, time lase imaging, heated stage and CO2 incubator.
- Fast image acquisition
Microscope | Olympus IX81, POC cell cultivation system, Cage incubator |
Objectives | 10X UPLFLN/NA 0.3 |
20X LUCPLFLN/NA 0.45 | |
60X PLAPO/TIRF/ NA 1.4 OIL | |
150X UAPO/ TIRF/NA 1.45 OIL | |
Lasers | 488nm, 561nm |
Epi-Filter cubes | 488/561 dual DAPI/FITC/TRITC CFP/YFP |
Excitation Filter (MT20) | 340 fura, 380 fura, 403 DAPI, 430 CFP, 492 FITC, 500 YFP, 572 TxRed |
Camera | EM CCD Hamamatsu |
Applications | TIRF/epi- fluorescence, fully automated multi-colour imaging, time series…. |
Software | Cell^R |