Biological Sciences

LEAP: Laser Enabled Analysis and Processing

Provides high-throughput microplate imaging of live cells in-situ, with laser-mediated cell processing capabilities

We are one of the few places in the UK with access to the versatile LEAP technology, which provides high-throughput microplate imaging of live cells in-situ, with laser-mediated cell processing capabilities. Cell populations can be selected by user-defined parameters, allowing elimination of unwanted cells, and optoinjection of a wide range of macromolecules into specific cell populations.

Brightfield imaging is LED-based, while multicolour fluorescence utilises a hybrid-halide lamp with 8 excitation and 8 emission filters, coupled to a CCD camera. Magnification is adjustable with 3x, 5x, 10x, and 20x available. Multi-wavelength imaging is integrated with software allowing cell characterisation and population selection by fluorescence intensity, size, granularity and shape.

Pulsed lasers are used only in cell processing (no imaging), with 2 wavelengths: 355nm (UV) & 532nm (Green). Laser targeting can be automated or manual, shooting up to 1000 cells/sec.

 

If you think the LEAP platform could assist with your research goals, please contact Dr. Caroline Wardropeto discuss your project:

 

UK Centre for Mammalian Synthetic Biology

3.11 CH Waddington Building

King’s Buildings

University of Edinburgh