What we offer
High-performance analysis and sorting capabilities
Flow cytometry is a laser-based technology that allows the multi-parameter measurement of characteristics of biological particles, in a single cell suspension, as they flow in a liquid medium past an excitation light source. This can be applied to whole cells as well as prepared cellular components such as nuclei or organelles. The underlying principle is that light is scattered and fluorescence is emitted as light from the excitation source strikes individual moving particles. Flow cytometry is particularly important for biological research because it allows qualitative and quantitative examination of whole cells and cellular constituents that have been labelled with a wide range of commercially available reagents, such as dyes, reporter proteins and monoclonal antibodies.
We have 20 parameter multiplexing capability with aseptic cell sorting technologies, including 4-way simultaneously sorting and single cell cloning.
The facility also offers HTS plate acquisition with support for experimental design and analysis.
Three Bench top analysers are available for multiparameter analysis:
FACSCalibur (BD) - dual laser - 488/633nm - 6 parameter
FACSCanto II (BD) - 407/488/633nm - 2/4/2 colour
LSR II (BD) - 355/407/488/633nm - 2/6/6/3 colour
LSRFortessa(SORP-BD)- 407/488/561/633nm - 6/2/5/3 colour
Also available is a state-of-the-art High speed cell sorter:
FACSAria IIu cell sorter - 407/488/561/633nm - 6/2/5/3 colour
The FACSAria Cell Sorter provides a method for dissecting a heterogenous mixture of biological cells into discreet sub-populations, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. It is a useful scientific instrument as it provides fast, objective and quantitative recording of fluorescent signals from individual cells as well as physical separation of cells of particular interest.