Long Read Sequencing
The facility operates the PacBio Sequel IIe and the Oxford Nanopore PromethION for long read sequencing.
Long read sequencing platforms
The Sequel IIe and PromethION are capable of generating many tens of gigabases per day. These data can be:
- assembled de novo to predict the sequence of a new genome or full length transcriptome
- aligned to a reference genome to identify single nucleotide, insertion-deletion polymorphisms, and structural variants
- the PromethION can generate RNA sequence directly
We can multiplex many samples per run, making the technologies very flexible.
We work closely with PacBio and Oxford Nanopore to offer the best long read technologies for your research.
Technologies
PacBio Sequel IIe
The PacBio Sequel IIe is based on the RSII and Sequel single molecule, real-time technology and delivers the highly accurate long HiFi reads. This platform delivers long reads, high consensus accuracy with HiFi, uniform coverage and epigenetic characterisation and is ideal for generating high-quality whole genome de novo assemblies. Edinburgh Genomics is proud to be the first facility in the Europe to be able to offer the Sequel IIe to the sequencing community. Contact us to take advantage of this exciting long-read technology for applications including De novo genome assemblies, structural variation, full length transcripts, long amplicons and real time methylome detection.
Oxford Nanopopre PromethION
The ONT PromethION offers real-time, long-read, direct DNA and RNA sequencing technology at a large scale. It offers the same pore-based sequencing as the MinION, but with many more pores for a much higher throughput and lower cost per base.
See also the long read sequencing page for Oxford nanopore and PacBio based sequencing