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Mistletoe under spotlight of major DNA study

It has been a frivolous part of Christmas festivities for centuries, but now scientists sense that untangling mistletoe’s complex make-up could lead to lasting benefits.

Edinburgh scientists are set to be the first to sequence the plant’s genome – the sum total of its entire DNA – which is more than forty times the size of the human genome.

The mistletoe genome will contribute to the Darwin Tree of Life Project, which aims to sequence the genomes of over 60,000 British and Irish species within the next 10 years.  

Mistletoe
Image credit: Getty images, aqua_tranquilla

The research is pioneering the use of ground-breaking gene sequencing technology, that could also be used to better understand diseases and cancers in humans and animals.

PacBio Sequel IIe System

Researchers at the University’s Edinburgh Genomics facility will be one of the first in Europe to use the PacBio Sequel IIe System, which is designed to read long fragments of DNA from virtually any species, with extremely high accuracy.

The system produces eight-times more data than earlier sequencers, making sequencing complex genomes more affordable. Experts will use the technology to rapidly decode the mistletoe’s entire DNA.

The results could reveal how mistletoe has evolved to become a parasite – in order to survive it attaches itself to host trees and feeds off them.

Project partners, the Royal Botatanic Garden Edinburgh, will provide the mistletoe sample, collected from near the Scottish Gallery of Modern Art.

Dr Javier Santoyo-Lopez, Service Manager at Edinburgh Genomics sequencing facility, said: “PacBio’s Sequel IIe System is a very powerful addition to our battery of sequencers at Edinburgh Genomics, enhancing the sequencing services we provide to researchers at the University at the same time as reducing costs.

Its capability to accurately read large fragments of DNA will allow us to fully characterize the genetic information of many organisms, such as the mistletoe, as well as detect complex genomic alterations that could be the cause of rare diseases or cancers.”

We’re excited to be the first to attempt to sequence the complex genome of the mistletoe, using the new PacBio Sequel IIe System. The results will become part of the Darwin Tree of Life Project, to sequence the genomes of all 60,000 British and Irish species within the next ten years.

 Dr Alex TwyfordLecturer in Botany at the School of Biological Sciences and Darwin Tree of Life University lead

Jonas Korlach, PhD, Chief Scientific Officer of PacBio, said: “We are proud to support the Darwin Tree of Life project and excited to see the complexity of a festive holiday plant like mistletoe revealed with HiFi reads. Projects and initiatives such as these are vital to generating complete reference genomes that advance research focused on understanding and preserving Earth’s biodiversity.”

This equipment was funded by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC). The Darwin Tree of Life project is funded by Wellcome.

Related Links

Edinburgh Genomics

The Darwin Tree of Life project