Biological Sciences

Events and seminars

Monday Seminar Series - "Transcription of the genome: mechanisms and regulatory principles"

Professor Patrick Cramer - Max Planck Institute for Biophysical Chemistry, MPG

2nd March 2020 at 12:15pm [Download iCalendar / .ics file]

Daniel Rutherford Building, G.27, Lecture Theatre

Our laboratory combines integrated structural biology with functional genomics and computational biology to study the mechanisms of gene transcription and its regulation in eukaryotic cells. In a long-term effort, we have now arrived at a molecular-mechanistic understanding of many aspects of transcription by RNA polymerase II. In particular, we reported insights into the mechanisms of transcription initiation at promoters of protein-coding genes (Plaschka et al. Nature 2015, 2016; Nozawa et al. Nature 2017; Schilbach et al. Nature 2017). We also recently reported the molecular basis for understanding transcription regulation at the step of elongation of the mRNA chain in the promoter-proximal region (Vos and Farnung Nature 2018; Vos et al. Nature 2018) and through chromatin (Farnung Nature 2017). These processes depend on multiple phosphorylation events, and such phosphorylation may control the partitioning of RNA polymerase II between different nuclear condensates (Boehning et al., NSMB 2018). To complement the structural studies, we also developed transient transcriptome sequencing (TT-seq), which monitors RNA synthesis and regulatory enhancer landscapes at high temporal resolution (Schwalb Science 2016; Demel Mol. Syst. Biol. 2017). In recent work, we have combined functional genomics and kinetic modeling to derive kinetic insights into transcriptonal regulation genome-wide (Gressel, Schwalb et al. Leonhardt, Eick, and Cramer, eLife 2017). In my lecture I will concentrate on the most recent findings and suggest out future directions for the investigation of the molecular and cellular mechanisms of genome regulation. A focus will be on the mechanisms of chromatin opening to generate nucleosome-depleted, transcriptionally active promoter regions at the beginning of genes.

Host JP Arulanandam

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